Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 32298..32890 Replicon plasmid unnamed2
Accession NZ_CP070231
Organism Escherichia coli strain EC12

Toxin (Protein)


Gene name hicA Uniprot ID A0A1I9ZJC5
Locus tag JTC67_RS25965 Protein ID WP_000513532.1
Coordinates 32642..32890 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1I9ZJ49
Locus tag JTC67_RS25960 Protein ID WP_000681609.1
Coordinates 32298..32645 (-) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTC67_RS25915 (JTC67_25730) 27589..27930 - 342 WP_000730722.1 plasmid mobilization relaxosome protein MobC -
JTC67_RS25920 (JTC67_25735) 28299..28700 + 402 WP_021575837.1 hypothetical protein -
JTC67_RS25925 (JTC67_25740) 28718..29470 + 753 WP_000797966.1 conjugal transfer protein TraL -
JTC67_RS25930 (JTC67_25745) 29472..30179 + 708 WP_000108794.1 hypothetical protein -
JTC67_RS25935 30199..30339 + 141 Protein_26 hypothetical protein -
JTC67_RS25940 (JTC67_25750) 30316..30639 + 324 WP_223666488.1 hypothetical protein -
JTC67_RS25945 (JTC67_25755) 30641..30967 + 327 WP_000206697.1 hypothetical protein -
JTC67_RS25950 (JTC67_25760) 31227..31526 - 300 WP_233679829.1 type II toxin-antitoxin system HicB family antitoxin -
JTC67_RS25955 31767..32210 + 444 WP_023223351.1 hypothetical protein -
JTC67_RS25960 (JTC67_25770) 32298..32645 - 348 WP_000681609.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JTC67_RS25965 (JTC67_25775) 32642..32890 - 249 WP_000513532.1 type II toxin-antitoxin system HicA family toxin Toxin
JTC67_RS25970 (JTC67_25780) 33355..33633 - 279 WP_000545937.1 hypothetical protein -
JTC67_RS25975 (JTC67_25785) 34183..34434 - 252 WP_001132407.1 transcriptional regulator -
JTC67_RS25980 (JTC67_25790) 34424..34684 - 261 WP_000702245.1 hypothetical protein -
JTC67_RS25985 (JTC67_25795) 34736..34960 - 225 WP_000814383.1 hypothetical protein -
JTC67_RS25990 (JTC67_25800) 35058..35267 - 210 WP_000866037.1 hypothetical protein -
JTC67_RS25995 (JTC67_25805) 35308..35631 - 324 WP_233679830.1 hypothetical protein -
JTC67_RS26000 (JTC67_25810) 36212..36541 - 330 WP_000855121.1 hypothetical protein -
JTC67_RS26005 (JTC67_25815) 37139..37804 + 666 WP_000532148.1 division plane positioning ATPase MipZ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..58414 58414


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9378.03 Da        Isoelectric Point: 10.4800

>T193572 WP_000513532.1 NZ_CP070231:c32890-32642 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Download         Length: 249 bp

>T193572 NZ_CP093030:c4998462-4998163 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13206.97 Da        Isoelectric Point: 4.8313

>AT193572 WP_000681609.1 NZ_CP070231:c32645-32298 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA

Download         Length: 348 bp

>AT193572 NZ_CP093030:c4998166-4997876 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9ZJC5

Antitoxin

Source ID Structure
AlphaFold DB A0A1I9ZJ49

References