Detailed information of TA system TA193566

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 15988..16513
• Replicon: plasmid unnamed1
• Accession: NZ_CP070230
• Organism: Escherichia coli strain EC12

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1PFV8
• Locus tag: JTC67_RS24920
• Protein ID: WP_001159871.1
• Coordinates: 15988..16293 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: JTC67_RS24925
• Protein ID: WP_062860119.1
• Coordinates: 16295..16513 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JTC67_RS24875 (11323)	11323..12345	+	1023	Protein_18	alpha-galactosidase	-
JTC67_RS24880 (12408)	12408..12521	+	114	Protein_19	MFS transporter	-
JTC67_RS24885 (12516)	12516..12815	-	300	Protein_20	integrase core domain-containing protein	-
JTC67_RS24890 (12814)	12814..13269	+	456	Protein_21	IS3 family transposase	-
JTC67_RS24895 (13302)	13302..13844	+	543	Protein_22	IS3-like element ISEc48 family transposase	-
JTC67_RS24900 (14032)	14032..14595	+	564	WP_062860118.1	hypothetical protein	-
JTC67_RS24905 (14850)	14850..15503	-	654	Protein_24	IS3 family transposase	-
JTC67_RS24910 (15512)	15512..15685	+	174	Protein_25	IS91 family transposase	-
JTC67_RS24915 (15855)	15855..15987	-	133	Protein_26	phage integrase family protein	-
JTC67_RS24920 (15988)	15988..16293	-	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	Toxin
JTC67_RS24925 (16295)	16295..16513	-	219	WP_062860119.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
JTC67_RS24930 (17109)	17109..17353	+	245	Protein_29	hypothetical protein	-
JTC67_RS24935 (17353)	17353..17943	+	591	WP_062860120.1	hypothetical protein	-
JTC67_RS24945 (19718)	19718..20533	+	816	WP_062860059.1	helix-turn-helix transcriptional regulator	-
JTC67_RS24950 (20561)	20561..21052	-	492	Protein_33	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	bfpK / bfpJ / bfpI / bfpH / bfpP / bfpF / bfpE / bfpD / bfpU / bfpC / bfpB / bfpG / bfpA	1..163194	163194

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11692.49 Da
• Isoelectric Point: 6.4674

>T193566 WP_001159871.1 NZ_CP070230:c16293-15988 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T193566 NZ_CP093030:c142402-142121 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8402.37 Da
• Isoelectric Point: 4.5203

>AT193566 WP_062860119.1 NZ_CP070230:c16513-16295 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVAWFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT193566 NZ_CP093030:c142626-142399 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• AlphaFold DB: A0A829CCE8