Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 4031035..4031618 Replicon chromosome
Accession NZ_CP070229
Organism Escherichia coli strain EC12

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag JTC67_RS20305 Protein ID WP_024232025.1
Coordinates 4031283..4031618 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag JTC67_RS20300 Protein ID WP_000581937.1
Coordinates 4031035..4031283 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTC67_RS20290 (4027374) 4027374..4028675 + 1302 WP_062860025.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
JTC67_RS20295 (4028723) 4028723..4030957 + 2235 WP_000226815.1 GTP pyrophosphokinase -
JTC67_RS20300 (4031035) 4031035..4031283 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
JTC67_RS20305 (4031283) 4031283..4031618 + 336 WP_024232025.1 endoribonuclease MazF Toxin
JTC67_RS20310 (4031690) 4031690..4032481 + 792 WP_001071670.1 nucleoside triphosphate pyrophosphohydrolase -
JTC67_RS20315 (4032709) 4032709..4034346 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
JTC67_RS20320 (4034434) 4034434..4035732 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 11968.82 Da        Isoelectric Point: 7.7127

>T193563 WP_024232025.1 NZ_CP070229:4031283-4031618 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWGARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T193563 NZ_CP093028:c5969532-5969254 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGCTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT193563 WP_000581937.1 NZ_CP070229:4031035-4031283 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT193563 NZ_CP093028:c5969242-5968937 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References