Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3913188..3913842 Replicon chromosome
Accession NZ_CP070229
Organism Escherichia coli strain EC12

Toxin (Protein)


Gene name cptA Uniprot ID A0A2A7MB46
Locus tag JTC67_RS19820 Protein ID WP_000244775.1
Coordinates 3913435..3913842 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JTC67_RS19815 Protein ID WP_000354046.1
Coordinates 3913188..3913454 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTC67_RS19795 (3909276) 3909276..3910709 - 1434 WP_001553397.1 6-phospho-beta-glucosidase BglA -
JTC67_RS19800 (3910754) 3910754..3911065 + 312 WP_001357616.1 N(4)-acetylcytidine aminohydrolase -
JTC67_RS19805 (3911229) 3911229..3911888 + 660 WP_000250274.1 hemolysin III family protein -
JTC67_RS19810 (3911965) 3911965..3912945 - 981 WP_001357615.1 tRNA-modifying protein YgfZ -
JTC67_RS19815 (3913188) 3913188..3913454 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JTC67_RS19820 (3913435) 3913435..3913842 + 408 WP_000244775.1 protein YgfX Toxin
JTC67_RS19825 (3913882) 3913882..3914403 - 522 WP_001055874.1 flavodoxin FldB -
JTC67_RS19830 (3914515) 3914515..3915411 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JTC67_RS19835 (3915436) 3915436..3916146 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JTC67_RS19840 (3916152) 3916152..3917885 + 1734 WP_001357613.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16008.94 Da        Isoelectric Point: 10.9373

>T193562 WP_000244775.1 NZ_CP070229:3913435-3913842 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLCLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T193562 NZ_CP093028:c5698937-5698527 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTTGACACCAACATCTGCATCTTCACCATCAAAAAACGTCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCATCACGACCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGACGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATCGCCGGGCACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGATTGGGTCACCC
CAATCGTATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT193562 WP_000354046.1 NZ_CP070229:3913188-3913454 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT193562 NZ_CP093028:c5699167-5698937 [Pseudomonas aeruginosa]
GTGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCCGTCGCGCTTCCTGATGACGT
TAAGCGTGTGGATGTGGTCGCTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGTGAGTCTTGGGATAGCTGGTTCGATG
GCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A7MB46

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References