Detailed information of TA system TA193561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 3359538..3360292
• Replicon: chromosome
• Accession: NZ_CP070229
• Organism: Escherichia coli strain EC12

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A822TTG1
• Locus tag: JTC67_RS16980
• Protein ID: WP_021560068.1
• Coordinates: 3359538..3360023 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: JTC67_RS16985
• Protein ID: WP_000801915.1
• Coordinates: 3360014..3360292 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JTC67_RS16955 (3354810)	3354810..3355640	+	831	WP_000928734.1	rhomboid family intramembrane serine protease GlpG	-
JTC67_RS16960 (3355657)	3355657..3356415	+	759	WP_001296480.1	DeoR/GlpR family transcriptional regulator	-
JTC67_RS16965 (3356397)	3356397..3357011	-	615	Protein_3317	DNA-binding transcriptional regulator RtcR	-
JTC67_RS16970 (3357230)	3357230..3358456	+	1227	WP_001105471.1	RNA-splicing ligase RtcB	-
JTC67_RS16975 (3358460)	3358460..3359488	+	1029	WP_024231689.1	RNA 3'-terminal phosphate cyclase	-
JTC67_RS16980 (3359538)	3359538..3360023	-	486	WP_021560068.1	GNAT family N-acetyltransferase	Toxin
JTC67_RS16985 (3360014)	3360014..3360292	-	279	WP_000801915.1	DUF1778 domain-containing protein	Antitoxin
JTC67_RS16990 (3360359)	3360359..3363064	-	2706	WP_000906938.1	HTH-type transcriptional regulator MalT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17680.60 Da
• Isoelectric Point: 9.4066

>T193561 WP_021560068.1 NZ_CP070229:c3360023-3359538 [Escherichia coli]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSSLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E

Nucleotide

• Length: 486 bp

>T193561 NZ_CP093028:c5312883-5312536 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGCGGCAGTGATCTTGGTTCTGCGACAGAAGCAGAGTGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10450.97 Da
• Isoelectric Point: 4.6336

>AT193561 WP_000801915.1 NZ_CP070229:c3360292-3360014 [Escherichia coli]
MKPESKETPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFILDDEQYDAFLAALDAPITAERQAK
INALMNRKSPWE

Nucleotide

• Length: 279 bp

>AT193561 NZ_CP093028:c5313144-5312893 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGACTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Structures

Toxin

• AlphaFold DB: A0A822TTG1