Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 3317307..3317902 Replicon chromosome
Accession NZ_CP070229
Organism Escherichia coli strain EC12

Toxin (Protein)


Gene name doc Uniprot ID A0A826P6U8
Locus tag JTC67_RS16785 Protein ID WP_000155160.1
Coordinates 3317525..3317902 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A827G8B6
Locus tag JTC67_RS16780 Protein ID WP_000557316.1
Coordinates 3317307..3317528 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTC67_RS16755 (3312383) 3312383..3313492 + 1110 WP_001297972.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
JTC67_RS16760 (3313540) 3313540..3314466 + 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
JTC67_RS16765 (3314463) 3314463..3315740 + 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
JTC67_RS16770 (3315737) 3315737..3316504 + 768 WP_000084170.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
JTC67_RS16775 (3316506) 3316506..3317219 + 714 WP_000416895.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
JTC67_RS16780 (3317307) 3317307..3317528 + 222 WP_000557316.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JTC67_RS16785 (3317525) 3317525..3317902 + 378 WP_000155160.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
JTC67_RS16790 (3318111) 3318111..3319427 + 1317 WP_000803589.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
JTC67_RS16795 (3319525) 3319525..3320412 + 888 WP_000099282.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
JTC67_RS16800 (3320409) 3320409..3321254 + 846 WP_000572183.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
JTC67_RS16805 (3321256) 3321256..3322326 + 1071 WP_001357972.1 sn-glycerol 3-phosphate ABC transporter ATP binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3314463..3323066 8603


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-53)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13945.20 Da        Isoelectric Point: 6.4830

>T193560 WP_000155160.1 NZ_CP070229:3317525-3317902 [Escherichia coli]
MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNKYEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGLILAANPEFVELTVDVAAGKVSLEDIVTRLRQHGT

Download         Length: 378 bp

>T193560 NZ_CP093028:c4849271-4848516 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATGTCCGACCGCTCCAGGGGCGGCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGTTGGGGG
TCGCGGTTTGGCCGTCGTCACGAGCCAAGCCTGTTCTACGCCGCGTTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGTGTCCTGTGGAGTGGCATGGCGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGGAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCTGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8106.25 Da        Isoelectric Point: 5.5759

>AT193560 WP_000557316.1 NZ_CP070229:3317307-3317528 [Escherichia coli]
MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEFERYQKAKLDAEFDAIMAIHGSEIMELADK

Download         Length: 222 bp

>AT193560 NZ_CP093028:c4849774-4849406 [Pseudomonas aeruginosa]
ATGAGCGCTCTCATCAAGGAACGTCCCAGCGCCGATGCTGTCCTGGCCAAGGCCGTCCTGGCCGCGCGCGAGCAATTGGG
GCTGACGCAGCTCGAACTGGCCGGCATCGTCGGCGTCGATCGCAGCGCCATCAGCCGCTGGAAGACCCAGGGCCTGCGGG
TGGACAGCAAGACCGGCGAGCTGGCTCTGCTGCTGGTGCGGGTCTATCGCGCACTGTATGCCCTGTTCGGCGGGCAGCAG
GAGGACATGCGCCACTTCCTGCGCACTCCCAACCATCACCTGGCGGGCGAGCCGCTGGCACTGATGGGACAGGTGCAGGG
CCTGGTTCATGTGCTGGAATACCTCGATGCGATCCGCGGCAAGGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A826P6U8

Antitoxin

Source ID Structure
AlphaFold DB A0A827G8B6

References