Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 1053892..1054363 Replicon chromosome
Accession NZ_CP070229
Organism Escherichia coli strain EC12

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag JTC67_RS05650 Protein ID WP_001303511.1
Coordinates 1054085..1054363 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID A0A1C7CIC8
Locus tag JTC67_RS05645 Protein ID WP_032300357.1
Coordinates 1053892..1054083 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTC67_RS05610 (1049100) 1049100..1049450 + 351 WP_000624677.1 IS66 family insertion sequence element accessory protein TnpB -
JTC67_RS05615 (1049480) 1049480..1051070 + 1591 Protein_1113 IS66 family transposase -
JTC67_RS05620 (1051097) 1051097..1051495 - 399 WP_233679788.1 DUF977 family protein -
JTC67_RS05625 (1051536) 1051536..1052606 - 1071 WP_074448915.1 phage replisome organizer -
JTC67_RS05630 (1052678) 1052678..1053103 - 426 WP_062897995.1 toxin YdaT family protein -
JTC67_RS05635 (1053100) 1053100..1053402 - 303 WP_000172735.1 transcriptional regulator -
JTC67_RS05640 (1053500) 1053500..1053871 + 372 WP_122993177.1 hypothetical protein -
JTC67_RS05645 (1053892) 1053892..1054083 + 192 WP_032300357.1 hypothetical protein Antitoxin
JTC67_RS05650 (1054085) 1054085..1054363 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JTC67_RS05655 (1054716) 1054716..1055015 + 300 WP_044705881.1 hypothetical protein -
JTC67_RS05660 (1055087) 1055087..1055305 + 219 WP_001171942.1 protein YdfC -
JTC67_RS05665 (1055328) 1055328..1055735 + 408 WP_074448913.1 hypothetical protein -
JTC67_RS05670 (1055713) 1055713..1055946 - 234 WP_000920491.1 hypothetical protein -
JTC67_RS05675 (1056512) 1056512..1056733 + 222 WP_000560212.1 cell division protein FtsZ -
JTC67_RS05680 (1056857) 1056857..1059337 + 2481 WP_074448912.1 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - espFu/tccP 1017621..1060849 43228


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T193551 WP_001303511.1 NZ_CP070229:1054085-1054363 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T193551 NZ_CP093023:c145583-145302 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGAAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7491.48 Da        Isoelectric Point: 8.7088

>AT193551 WP_032300357.1 NZ_CP070229:1053892-1054083 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFARMRKERSKQ

Download         Length: 192 bp

>AT193551 NZ_CP093023:c145807-145580 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAAAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
AlphaFold DB A0A1C7CIC8

References