Detailed information of TA system TA193550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 919925..920709
• Replicon: chromosome
• Accession: NZ_CP070229
• Organism: Escherichia coli strain EC12

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: JTC67_RS04890
• Protein ID: WP_000613626.1
• Coordinates: 920215..920709 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: JTC67_RS04885
• Protein ID: WP_062859998.1
• Coordinates: 919925..920218 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JTC67_RS04875 (915074)	915074..916033	-	960	WP_000846343.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
JTC67_RS04880 (916606)	916606..919791	+	3186	WP_001357075.1	ribonuclease E	-
JTC67_RS04885 (919925)	919925..920218	+	294	WP_062859998.1	DUF1778 domain-containing protein	Antitoxin
JTC67_RS04890 (920215)	920215..920709	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
JTC67_RS04895 (920804)	920804..921757	-	954	WP_001212764.1	flagellar hook-associated protein FlgL	-
JTC67_RS04900 (921769)	921769..923412	-	1644	WP_001357076.1	flagellar hook-associated protein FlgK	-
JTC67_RS04905 (923478)	923478..924419	-	942	WP_001304468.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
JTC67_RS04910 (924419)	924419..925516	-	1098	WP_000589300.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-95)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T193550 WP_000613626.1 NZ_CP070229:920215-920709 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T193550 NZ_CP093022:c5787461-5787183 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGTGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11118.70 Da
• Isoelectric Point: 10.5423

>AT193550 WP_062859998.1 NZ_CP070229:919925-920218 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDHPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT193550 NZ_CP093022:c5787171-5786866 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAATGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCAGGGATAG

Structures

Toxin

• AlphaFold DB: V0T0H9