Detailed information of TA system TA193548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 536197..537785
• Replicon: chromosome
• Accession: NZ_CP070229
• Organism: Escherichia coli strain EC12

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: JTC67_RS03000
• Protein ID: WP_021559882.1
• Coordinates: 536463..537785 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: JTC67_RS02995
• Protein ID: WP_001357336.1
• Coordinates: 536197..536463 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JTC67_RS02980 (532565)	532565..534016	+	1452	WP_000854637.1	tagaturonate reductase	-
JTC67_RS02985 (534213)	534213..535127	+	915	WP_001313828.1	bestrophin family protein	-
JTC67_RS02990 (535131)	535131..535889	-	759	WP_001286544.1	trans-aconitate 2-methyltransferase	-
JTC67_RS02995 (536197)	536197..536463	+	267	WP_001357336.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JTC67_RS03000 (536463)	536463..537785	+	1323	WP_021559882.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
JTC67_RS03005 (538161)	538161..538343	-	183	WP_021559714.1	hypothetical protein	-
JTC67_RS03010 (538458)	538458..540458	+	2001	WP_233679767.1	replication endonuclease	-
JTC67_RS03015 (540489)	540489..540914	+	426	WP_233679768.1	hypothetical protein	-
JTC67_RS03020 (541052)	541052..541222	+	171	WP_000134225.1	hypothetical protein	-
JTC67_RS03025 (541248)	541248..541490	+	243	WP_074448977.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49298.00 Da
• Isoelectric Point: 8.1297

>T193548 WP_021559882.1 NZ_CP070229:536463-537785 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAAEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTLLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T193548 NZ_CP093022:c4937820-4937455 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGAGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCATG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCTGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCTATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10014.36 Da
• Isoelectric Point: 4.9632

>AT193548 WP_001357336.1 NZ_CP070229:536197-536463 [Escherichia coli]
MMNFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT193548 NZ_CP093022:c4937462-4937139 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGGCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCACGTGC
AGCAGCCGGCAATTGCCAAAATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATCGCACGCTTCCCGGATGGCGCGGTGAAAATCAGCAACTTCGCTGATATCGAAGAGGCGGGCGC
CTAA