Detailed information of TA system TA193537

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4173027..4173859
• Replicon: chromosome
• Accession: NZ_CP070227
• Organism: Escherichia coli strain JA0072

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: JS570_RS19770
• Protein ID: WP_000854753.1
• Coordinates: 4173485..4173859 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: JS570_RS19765
• Protein ID: WP_001278232.1
• Coordinates: 4173027..4173395 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JS570_RS19735 (4169863)	4169863..4170318	+	456	WP_000581504.1	IrmA family protein	-
JS570_RS19740 (4170397)	4170397..4170630	+	234	WP_001119729.1	DUF905 family protein	-
JS570_RS19745 (4170730)	4170730..4171548	+	819	WP_001520163.1	DUF932 domain-containing protein	-
JS570_RS19750 (4171603)	4171603..4172088	+	486	WP_001520165.1	antirestriction protein	-
JS570_RS19755 (4172104)	4172104..4172580	+	477	WP_001186726.1	RadC family protein	-
JS570_RS19760 (4172643)	4172643..4172864	+	222	WP_000692316.1	DUF987 domain-containing protein	-
JS570_RS19765 (4173027)	4173027..4173395	+	369	WP_001278232.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
JS570_RS19770 (4173485)	4173485..4173859	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
JS570_RS19775 (4173856)	4173856..4174344	+	489	WP_000777541.1	DUF5983 family protein	-
JS570_RS19780 (4174356)	4174356..4174553	+	198	WP_000839281.1	DUF957 domain-containing protein	-
JS570_RS19785 (4174650)	4174650..4175219	+	570	WP_001290241.1	DUF4942 domain-containing protein	-
JS570_RS19790 (4175561)	4175561..4175731	+	171	Protein_3885	IS110 family transposase	-
JS570_RS19795 (4176542)	4176542..4177525	+	984	WP_001616958.1	KpsF/GutQ family sugar-phosphate isomerase	-
JS570_RS19800 (4177597)	4177597..4178745	+	1149	WP_000905922.1	capsule polysaccharide export inner-membrane protein KpsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T193537 WP_000854753.1 NZ_CP070227:4173485-4173859 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T193537 NZ_CP093020:c5639726-5639448 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT193537 WP_001278232.1 NZ_CP070227:4173027-4173395 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT193537 NZ_CP093020:c5639436-5639131 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: E3PJ72