Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2517502..2519090 Replicon chromosome
Accession NZ_CP070227
Organism Escherichia coli strain JA0072

Toxin (Protein)


Gene name hipA Uniprot ID A0A192ENQ5
Locus tag JS570_RS12105 Protein ID WP_001125432.1
Coordinates 2517502..2518824 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID V0VAH2
Locus tag JS570_RS12110 Protein ID WP_001304368.1
Coordinates 2518824..2519090 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JS570_RS12070 (2513269) 2513269..2513502 - 234 WP_000473544.1 hypothetical protein -
JS570_RS12075 (2513537) 2513537..2513788 - 252 WP_000381239.1 membrane protein -
JS570_RS12080 (2513791) 2513791..2514108 - 318 WP_000073835.1 DNA-binding protein -
JS570_RS12085 (2514120) 2514120..2515172 - 1053 WP_000568109.1 phage/plasmid replication protein, II/X family -
JS570_RS12090 (2515344) 2515344..2515790 + 447 WP_000527682.1 helix-turn-helix transcriptional regulator -
JS570_RS12095 (2515890) 2515890..2516309 + 420 WP_000089315.1 hypothetical protein -
JS570_RS12100 (2516396) 2516396..2517325 + 930 WP_000963948.1 hypothetical protein -
JS570_RS12105 (2517502) 2517502..2518824 - 1323 WP_001125432.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
JS570_RS12110 (2518824) 2518824..2519090 - 267 WP_001304368.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
JS570_RS12115 (2519398) 2519398..2520156 + 759 WP_001286543.1 trans-aconitate 2-methyltransferase -
JS570_RS12120 (2520160) 2520160..2521074 - 915 WP_001313828.1 bestrophin family protein -
JS570_RS12125 (2521271) 2521271..2522722 - 1452 WP_000854614.1 tagaturonate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49297.02 Da        Isoelectric Point: 8.6972

>T193525 WP_001125432.1 NZ_CP070227:c2518824-2517502 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Download         Length: 1323 bp

>T193525 NZ_CP093016:c4616809-4616231 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTGTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTCCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAGGCCGCGCACCCGGACAAAGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCACCTCGACGC
GCCGCTGCGCGACCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGTCCGCCGAAGCCCTGGCAGTGAAGGCCC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGCGCCTCGC
AGTTCGGCGCGACGCCTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9957.31 Da        Isoelectric Point: 4.9632

>AT193525 WP_001304368.1 NZ_CP070227:c2519090-2518824 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Download         Length: 267 bp

>AT193525 NZ_CP093016:c4617452-4616898 [Pseudomonas aeruginosa]
ATGACCGAGCCTCTCCCTTCTTCGTCCCTGGGCCCCGCCCTGCGTCGCTGGCGCCTGCTGTACCGGGTCAAGCAAACGCA
TGCCGCCGAATTGCTCGGCGTGGCGCAATCGACCATCTCGCGCTGGGAGTCCGGTACCCAGGCGCTGCTGGTGGAGGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAGCCGCCGCCGACCAGGCACTGGCCCGCCTGGTCGAGGCCAAC
CCGCAACCCGTGCACCTGATCTGCGACCTCACCCATCGCCTGTTGGCCTGTTCGCCGGCACGCGCCGCGCAATTCGGCGT
GCCTCTCGGCGAACTGCTCGACCGTTCGCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAGCCACCCTCGAGG
AACTCGGCTGGCGCGAACTGCTGGCACCGCCGGCGCTGGAGTTCGCCAGCGGAGCGAACGCCTCGGCCATCGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACCCTCTCGGACGGACGCGCGGTGCGCCTGGTGGAAACCCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192ENQ5

Antitoxin

Source ID Structure
AlphaFold DB V0VAH2

References