Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 886348..886898 Replicon chromosome
Accession NZ_CP070227
Organism Escherichia coli strain JA0072

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag JS570_RS04110 Protein ID WP_001160966.1
Coordinates 886584..886898 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag JS570_RS04105 Protein ID WP_000125566.1
Coordinates 886348..886581 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JS570_RS04080 (881421) 881421..881708 + 288 WP_000203741.1 ferredoxin-like protein FixX -
JS570_RS04085 (881767) 881767..883098 + 1332 WP_001183207.1 MFS transporter -
JS570_RS04090 (883206) 883206..883736 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
JS570_RS04095 (883729) 883729..885591 + 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
JS570_RS04100 (885783) 885783..886262 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
JS570_RS04105 (886348) 886348..886581 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
JS570_RS04110 (886584) 886584..886898 + 315 WP_001160966.1 CcdB family protein Toxin
JS570_RS04115 (886895) 886895..887743 - 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
JS570_RS04120 (887750) 887750..888127 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
JS570_RS04125 (888130) 888130..888951 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
JS570_RS04130 (888948) 888948..889937 - 990 WP_000241228.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
JS570_RS04135 (889937) 889937..891223 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T193520 WP_001160966.1 NZ_CP070227:886584-886898 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T193520 NZ_CP093015:c5643040-5642762 [Pseudomonas aeruginosa]
CTGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTTGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGCTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT193520 WP_000125566.1 NZ_CP070227:886348-886581 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT193520 NZ_CP093015:c5642750-5642445 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAATGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCAGGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References