Detailed information of TA system TA193513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 53385..53910
• Replicon: plasmid unnamed2
• Accession: NZ_CP070174
• Organism: Klebsiella pneumoniae strain FDAARGOS_1315

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A9E1GGX2
• Locus tag: I6K45_RS27060
• Protein ID: WP_016946795.1
• Coordinates: 53605..53910 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: I6K45_RS27055
• Protein ID: WP_004197642.1
• Coordinates: 53385..53603 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K45_RS27015	48761..48964	-	204	WP_004197809.1	hypothetical protein	-
I6K45_RS27020	48978..49181	-	204	WP_097404640.1	hemolysin expression modulator Hha	-
I6K45_RS27025	49215..49583	-	369	WP_004197807.1	hypothetical protein	-
I6K45_RS27030	49627..50121	-	495	WP_020802777.1	hypothetical protein	-
I6K45_RS27035	50152..50727	-	576	WP_020317495.1	hypothetical protein	-
I6K45_RS27040	50715..50984	-	270	WP_020804424.1	hypothetical protein	-
I6K45_RS27045	51421..52110	+	690	WP_020315560.1	RES family NAD+ phosphorylase	-
I6K45_RS27050	52142..52831	-	690	WP_004193995.1	hypothetical protein	-
I6K45_RS27055	53385..53603	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
I6K45_RS27060	53605..53910	+	306	WP_016946795.1	type II toxin-antitoxin system toxin CcdB	Toxin
I6K45_RS27065	54068..54751	+	684	WP_023280892.1	hypothetical protein	-
I6K45_RS27070	54754..55530	+	777	WP_049245386.1	site-specific integrase	-
I6K45_RS27075	55588..55845	-	258	WP_000764642.1	hypothetical protein	-
I6K45_RS27080	55974..56078	-	105	WP_032409716.1	hypothetical protein	-
I6K45_RS27085	56613..57479	+	867	WP_004118283.1	replication initiation protein	-
I6K45_RS27090	57836..58105	-	270	WP_000339857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..68255	68255

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11548.22 Da
• Isoelectric Point: 5.6831

>T193513 WP_016946795.1 NZ_CP070174:53605-53910 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDDSYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T193513 NZ_CP093015:283144-283470 [Pseudomonas aeruginosa]
ATGAATGACCCCGTGTACTGCCCGGTTCTTGGGTATGACGCGGTGTTGCTTGTCAACATCTCGACTGTCTATCCAGATAA
GTATTTCGATCAGTCATGCATTCTAAAGAAGGGTTGTCATCCCTTTGTTCTGCACGACAGCTGGGTCGTATACCGCGAGG
CAGGCCCTTTCAACCTTGCACGGCTTGAGCAAGGGGTAGCGCAGAAGTCCATCAAGACGCATCAGCCCATCAACGCAGGC
GATTTCACTCGGATTCGGGCAGGCTTCGACATCTCGCCCGACGTCAAAATCAAAGTTGAGCGGTTCATGAGGCAGCACAA
GATTTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT193513 WP_004197642.1 NZ_CP070174:53385-53603 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT193513 NZ_CP093015:282515-283072 [Pseudomonas aeruginosa]
GTGATCTTCAGTGAACAGAAAGTGGCTCACATGGCAGCTTTCTTTTTGGCGAGTGCCGAAGGGCCGCTGCCTCATCTCAC
GCTGATCAAACTGCTGTACCTGGCAGACCGAGAGTCGATGGCTCGTTTCGACGTTCCCATGTCGGACGATCGAGCTGTTT
CCATGCCCCATGGGCCTGTCCTTTCCTCGACGCTAGACCTCATCAAGGGGCGTTTGCGCTCTGAGGTTTGGAGCGCTTGG
ATTTCTCCGTTGGTGGATCACGAGGTCCGTCTGGTCCATGACGTGGAGAATTGGGACGATCTGGATGAGTTGAGCCGATC
GGATCGCAGCATTTTGGAATCGGTCTGGCAAGAGTTTGGCCACATGACCAAGTACCAATTGCGGGACTACACCCACCATC
ACATTGGTGAGTGGCACGACCCCCACGGCTCTTCCAGGGACATTGATCCAAAGGATACTTTCATGGCGCTTGGTCAAAGC
GAGGAGCTGGCGGAGATCAAGGCGCGCGATCTTGCTGAGCGCCGTCGGCACGGTAGGAAGCTGTCTGAGTTCATTTGA