Detailed information of TA system TA193490

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 112467..113245
• Replicon: chromosome
• Accession: NZ_CP070172
• Organism: Klebsiella pneumoniae strain FDAARGOS_1315

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: I6K45_RS00570
• Protein ID: WP_031591140.1
• Coordinates: 112467..112955 (-)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: W8UEW1
• Locus tag: I6K45_RS00575
• Protein ID: WP_004150912.1
• Coordinates: 112952..113245 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K45_RS00550	108004..108261	-	258	WP_158236380.1	ash family protein	-
I6K45_RS00555	108344..109276	+	933	Protein_107	hypothetical protein	-
I6K45_RS00560	109349..110457	+	1109	WP_087859145.1	IS3-like element ISKpn20 family transposase	-
I6K45_RS00565	110473..112386	+	1914	Protein_109	hypothetical protein	-
I6K45_RS00570	112467..112955	-	489	WP_031591140.1	GNAT family N-acetyltransferase	Toxin
I6K45_RS00575	112952..113245	-	294	WP_004150912.1	DUF1778 domain-containing protein	Antitoxin
I6K45_RS00580	113890..115266	+	1377	WP_004181304.1	cystathionine beta-synthase	-
I6K45_RS00585	115277..116425	+	1149	WP_032442510.1	PLP-dependent aspartate aminotransferase family protein	-
I6K45_RS00590	116426..117337	-	912	WP_032417598.1	LysR family transcriptional regulator	-
I6K45_RS00595	117435..118037	+	603	WP_049190181.1	short chain dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-95)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17604.53 Da
• Isoelectric Point: 7.7754

>T193490 WP_031591140.1 NZ_CP070172:c112955-112467 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCGSDSNVLAYYSLASSAVMTNTAPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEAREFYLRVGFEPSPMDPMMLMVTLGDLV
HA

Nucleotide

• Length: 489 bp

>T193490 NZ_CP093011:3781211-3781462 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10901.46 Da
• Isoelectric Point: 6.3783

>AT193490 WP_004150912.1 NZ_CP070172:c113245-112952 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Nucleotide

• Length: 294 bp

>AT193490 NZ_CP093011:3781459-3781713 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GVL4