Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 5363..5922 | Replicon | plasmid unnamed |
| Accession | NZ_CP070171 | ||
| Organism | Escherichia coli strain FDAARGOS_1284 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | V0SVC8 |
| Locus tag | I6K14_RS23580 | Protein ID | WP_000421272.1 |
| Coordinates | 5363..5638 (-) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | V0SRR3 |
| Locus tag | I6K14_RS23585 | Protein ID | WP_001387467.1 |
| Coordinates | 5638..5922 (-) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| I6K14_RS23550 (1413) | 1413..2153 | - | 741 | WP_001066953.1 | tyrosine-type recombinase/integrase | - |
| I6K14_RS23555 (2274) | 2274..2456 | - | 183 | WP_042344623.1 | hypothetical protein | - |
| I6K14_RS24685 (2523) | 2523..2812 | - | 290 | Protein_3 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| I6K14_RS23560 (2799) | 2799..3067 | - | 269 | Protein_4 | type II toxin-antitoxin system ParD family antitoxin | - |
| I6K14_RS23565 (3599) | 3599..4768 | + | 1170 | WP_001072355.1 | hypothetical protein | - |
| I6K14_RS23570 (4801) | 4801..4929 | + | 129 | Protein_6 | IS481 family transposase | - |
| I6K14_RS23575 (5054) | 5054..5257 | - | 204 | Protein_7 | hypothetical protein | - |
| I6K14_RS23580 (5363) | 5363..5638 | - | 276 | WP_000421272.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| I6K14_RS23585 (5638) | 5638..5922 | - | 285 | WP_001387467.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| I6K14_RS23590 (6527) | 6527..7279 | + | 753 | WP_000562172.1 | IclR family transcriptional regulator | - |
| I6K14_RS23595 (7325) | 7325..8290 | - | 966 | WP_001022265.1 | 2-keto-3-deoxygluconate transporter | - |
| I6K14_RS23600 (8323) | 8323..8703 | - | 381 | WP_000710783.1 | Rid family detoxifying hydrolase | - |
| I6K14_RS23605 (8728) | 8728..9618 | - | 891 | WP_001077068.1 | dihydrodipicolinate synthase family protein | - |
| I6K14_RS23610 (9851) | 9851..10045 | - | 195 | WP_000796505.1 | hypothetical protein | - |
| I6K14_RS23615 (10019) | 10019..10459 | + | 441 | Protein_15 | zinc-binding dehydrogenase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | tet(B) / catA1 / dfrA8 / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / sitABCD | - | 1..118581 | 118581 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10749.45 Da Isoelectric Point: 10.0871
>T193482 WP_000421272.1 NZ_CP070171:c5638-5363 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T193482 NZ_CP093011:1542230-1542544 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA
Antitoxin
Download Length: 95 a.a. Molecular weight: 10460.87 Da Isoelectric Point: 5.6558
>AT193482 WP_001387467.1 NZ_CP070171:c5922-5638 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR
Download Length: 285 bp
>AT193482 NZ_CP093011:1542541-1542957 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | V0SVC8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0Y880 |