Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4004006..4004556 Replicon chromosome
Accession NZ_CP070170
Organism Escherichia coli strain FDAARGOS_1284

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag I6K14_RS19135 Protein ID WP_001160966.1
Coordinates 4004006..4004320 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag I6K14_RS19140 Protein ID WP_000125568.1
Coordinates 4004323..4004556 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K14_RS19110 (3999681) 3999681..4000967 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
I6K14_RS19115 (4000967) 4000967..4001956 + 990 WP_000241225.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
I6K14_RS19120 (4001953) 4001953..4002774 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
I6K14_RS19125 (4002777) 4002777..4003154 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
I6K14_RS19130 (4003161) 4003161..4004009 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
I6K14_RS19135 (4004006) 4004006..4004320 - 315 WP_001160966.1 CcdB family protein Toxin
I6K14_RS19140 (4004323) 4004323..4004556 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
I6K14_RS19145 (4004642) 4004642..4005121 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
I6K14_RS19150 (4005313) 4005313..4007175 - 1863 WP_000377109.1 glutathione-regulated potassium-efflux system protein KefC -
I6K14_RS19155 (4007168) 4007168..4007698 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
I6K14_RS19160 (4007806) 4007806..4009137 - 1332 WP_001183198.1 MFS transporter -
I6K14_RS19165 (4009196) 4009196..4009483 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T193479 WP_001160966.1 NZ_CP070170:c4004320-4004006 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T193479 NZ_CP093011:1137958-1138176 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT193479 WP_000125568.1 NZ_CP070170:c4004556-4004323 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT193479 NZ_CP093011:1137558-1137932 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References