Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3540642..3541260 Replicon chromosome
Accession NZ_CP070170
Organism Escherichia coli strain FDAARGOS_1284

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6K14_RS16870 Protein ID WP_001291435.1
Coordinates 3541042..3541260 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6K14_RS16865 Protein ID WP_000344800.1
Coordinates 3540642..3541016 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K14_RS16855 (3535731) 3535731..3536924 + 1194 WP_001538394.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I6K14_RS16860 (3536947) 3536947..3540096 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
I6K14_RS16865 (3540642) 3540642..3541016 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6K14_RS16870 (3541042) 3541042..3541260 + 219 WP_001291435.1 HHA domain-containing protein Toxin
I6K14_RS16875 (3541433) 3541433..3541984 + 552 WP_000102539.1 maltose O-acetyltransferase -
I6K14_RS16880 (3542100) 3542100..3542570 + 471 WP_000136192.1 YlaC family protein -
I6K14_RS16885 (3542734) 3542734..3544284 + 1551 WP_204653445.1 EAL domain-containing protein -
I6K14_RS16890 (3544326) 3544326..3544679 - 354 WP_000878140.1 DUF1428 domain-containing protein -
I6K14_RS16900 (3545058) 3545058..3545369 + 312 WP_000409908.1 MGMT family protein -
I6K14_RS16905 (3545400) 3545400..3545972 - 573 WP_087597809.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T193477 WP_001291435.1 NZ_CP070170:3541042-3541260 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T193477 NZ_CP093011:c777029-776927 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT193477 WP_000344800.1 NZ_CP070170:3540642-3541016 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT193477 NZ_CP093011:776889-777157 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References