Detailed information of TA system TA193476

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2875754..2876555
• Replicon: chromosome
• Accession: NZ_CP070170
• Organism: Escherichia coli strain FDAARGOS_1284

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0H2V687
• Locus tag: I6K14_RS13765
• Protein ID: WP_000854739.1
• Coordinates: 2875754..2876134 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: I6K14_RS13770
• Protein ID: WP_087597879.1
• Coordinates: 2876181..2876555 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K14_RS13730 (2871345)	2871345..2871899	-	555	WP_001001921.1	molecular chaperone YcdY	-
I6K14_RS13735 (2871923)	2871923..2872660	-	738	WP_000283664.1	zinc-binding phosphatase	-
I6K14_RS13740 (2872715)	2872715..2873653	-	939	WP_087597881.1	glyoxylate/hydroxypyruvate reductase GhrA	-
I6K14_RS13750 (2874124)	2874124..2874967	-	844	Protein_2730	DUF4942 domain-containing protein	-
I6K14_RS13755 (2875052)	2875052..2875249	-	198	WP_000772027.1	DUF957 domain-containing protein	-
I6K14_RS13760 (2875269)	2875269..2875757	-	489	WP_001054232.1	DUF5983 family protein	-
I6K14_RS13765 (2875754)	2875754..2876134	-	381	WP_000854739.1	TA system toxin CbtA family protein	Toxin
I6K14_RS13770 (2876181)	2876181..2876555	-	375	WP_087597879.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6K14_RS13775 (2876605)	2876605..2877249	-	645	WP_021522037.1	hypothetical protein	-
I6K14_RS13780 (2877268)	2877268..2877489	-	222	WP_000692315.1	DUF987 domain-containing protein	-
I6K14_RS13785 (2877552)	2877552..2878028	-	477	WP_001186193.1	RadC family protein	-
I6K14_RS13790 (2878044)	2878044..2878517	-	474	WP_001313575.1	antirestriction protein	-
I6K14_RS13795 (2878611)	2878611..2878856	-	246	WP_001164966.1	hypothetical protein	-
I6K14_RS13800 (2878856)	2878856..2879677	-	822	WP_096960685.1	DUF932 domain-containing protein	-
I6K14_RS24550 (2879856)	2879856..2879945	-	90	WP_112031555.1	DUF905 family protein	-
I6K14_RS13810 (2880088)	2880088..2880543	-	456	WP_000581502.1	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14214.14 Da
• Isoelectric Point: 6.4773

>T193476 WP_000854739.1 NZ_CP070170:c2876134-2875754 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNDITLGRHPEEAKQ

Nucleotide

• Length: 381 bp

>T193476 NZ_CP093011:c496055-494733 [Escherichia coli]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCAGCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAACGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGGCTCACACCATTTTACGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGATCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13817.48 Da
• Isoelectric Point: 4.5518

>AT193476 WP_087597879.1 NZ_CP070170:c2876555-2876181 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQYTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT193476 NZ_CP093011:c496321-496055 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAGTTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTGACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAAGCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0H2V687