Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1833933..1834764 Replicon chromosome
Accession NZ_CP070170
Organism Escherichia coli strain FDAARGOS_1284

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag I6K14_RS08640 Protein ID WP_000854814.1
Coordinates 1833933..1834307 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag I6K14_RS08645 Protein ID WP_001285586.1
Coordinates 1834396..1834764 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K14_RS08600 (1829330) 1829330..1830496 + 1167 WP_001297905.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
I6K14_RS08605 (1830614) 1830614..1831087 + 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
I6K14_RS08610 (1831285) 1831285..1832343 + 1059 WP_001200890.1 FUSC family protein -
I6K14_RS08615 (1832515) 1832515..1832844 + 330 WP_000450409.1 DUF496 family protein -
I6K14_RS08620 (1832945) 1832945..1833079 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
I6K14_RS08625 (1833181) 1833181..1833327 + 147 Protein_1709 transposase domain-containing protein -
I6K14_RS08630 (1833616) 1833616..1833696 - 81 Protein_1710 hypothetical protein -
I6K14_RS08635 (1833742) 1833742..1833936 - 195 WP_000988601.1 DUF5983 family protein -
I6K14_RS08640 (1833933) 1833933..1834307 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
I6K14_RS08645 (1834396) 1834396..1834764 - 369 WP_001285586.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
I6K14_RS08650 (1834838) 1834838..1835059 - 222 WP_000692323.1 DUF987 domain-containing protein -
I6K14_RS08655 (1835128) 1835128..1835604 - 477 WP_122985903.1 RadC family protein -
I6K14_RS08660 (1835620) 1835620..1836093 - 474 WP_000855074.1 antirestriction protein -
I6K14_RS08665 (1836356) 1836356..1837177 - 822 WP_001234571.1 DUF932 domain-containing protein -
I6K14_RS08670 (1837398) 1837398..1837808 - 411 WP_000846704.1 hypothetical protein -
I6K14_RS08675 (1837824) 1837824..1838501 - 678 WP_001362823.1 hypothetical protein -
I6K14_RS08680 (1838637) 1838637..1839707 - 1071 WP_000102631.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T193465 WP_000854814.1 NZ_CP070170:c1834307-1833933 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T193465 NZ_CP092988:c43384-43136 [Leptospira interrogans]
ATGAAGGCTTCAGTCGTAAAAATAGGCAATTCCAAAGGTATTCGAATTCCCAAGGCAGTCTTAGAAGAATGTCATATTGA
AGAAGAAGTAGATTTACTAATCGATAATAACAAATTAATTATTGTACCTCTAAAATCGAAACCAAGAGAAGGGTGGGAAA
AACAATTTAAAACAATGTCATCTAATAAAGATGATAAATTATTTATTCCTGATTCAATAGATTTATTAGACAAGGATTGG
GAATGGTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT193465 WP_001285586.1 NZ_CP070170:c1834764-1834396 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT193465 NZ_CP092988:c43142-42819 [Leptospira interrogans]
ATGGTGATTTCTCAATACGAAGTATATCTAATAAATTTAGATCCAACGGTTGGACATGAGATAAAAAAATCAAGACCTTG
TGCAATAATCTCACCCAATGAAATGAATAAAACGATTGGAACAATTATTATTGCACCAATGACTACAAAATCTCGATCAT
ATCCGACAAGAGTTGAATTAACCTTTCAAGGAAAGAAGGGTTGGATTGTGTTAGATCAGATTCGAGCTGTTGATAAAACA
CGTCTGGTAAAGAAGTTAGGAAAAATAGATCCCAAAACAGTTAATAAAATGAAACTGGTAATAAAGGAAATGTTGGTTGA
TTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References