Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1013726..1014309 Replicon chromosome
Accession NZ_CP070170
Organism Escherichia coli strain FDAARGOS_1284

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag I6K14_RS04745 Protein ID WP_000254750.1
Coordinates 1013974..1014309 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag I6K14_RS04740 Protein ID WP_000581937.1
Coordinates 1013726..1013974 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K14_RS04730 (1010065) 1010065..1011366 + 1302 WP_000046817.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
I6K14_RS04735 (1011414) 1011414..1013648 + 2235 WP_000226815.1 GTP pyrophosphokinase -
I6K14_RS04740 (1013726) 1013726..1013974 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
I6K14_RS04745 (1013974) 1013974..1014309 + 336 WP_000254750.1 endoribonuclease MazF Toxin
I6K14_RS04750 (1014381) 1014381..1015172 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
I6K14_RS04755 (1015400) 1015400..1017037 + 1638 WP_021531536.1 CTP synthase (glutamine hydrolyzing) -
I6K14_RS04760 (1017125) 1017125..1018423 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T193462 WP_000254750.1 NZ_CP070170:1013974-1014309 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T193462 NZ_CP092985:1859797-1860138 [Leptospira interrogans]
ATGATTCGTGGTGAAATTTGGTGGGTAGACTTGGGAATTCCATTTGGAAGTGAGCCTGGATTTAAACGTCCAGTTCTTAT
AATTCAAGATGACTCTTTTAATCAAAGTAATATTAATACAATAGTTTCGATTGCAATTACATCGAATTTAAATCTATCTG
AAGCACCAGGTAATGTTTTCATTAGTAAAAAAGATTCGAGTTTATCAAAGGACTCGGTCATAAATGTTTCTCAAATTGTG
ACTTTAGATAAAGAAAGATTTCTTAACAAAGCGGGTAAACTTAAATCAAATAAATTAGGTGAAGTTGAAATTGGGCTTAA
GTTGGTTACAGGCTTAGATTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT193462 WP_000581937.1 NZ_CP070170:1013726-1013974 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT193462 NZ_CP092985:1859562-1859810 [Leptospira interrogans]
ATGAAGACGGCGATTTCAATTCCTGATGAGTTATTTAGGGCTGCCGAGAAAATAGCTAAAAAACTTGGGATTCCTCGAAG
CCAGTTATTTGCAAAAGCATTAGAAGAATTTATCCAATCTCATAGTAAAGAATCTGTAACTGAGAAATTAAATAAAATCT
ACAGTAATAAATCCAAAGAAACTAGAAATAATATTACTGACTTATCCGTAGAATCGCTTCGTAAGAGTTTAAAAAATGAT
TCGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References