Detailed information of TA system TA193460

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 712230..713062
• Replicon: chromosome
• Accession: NZ_CP070170
• Organism: Escherichia coli strain FDAARGOS_1284

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: I6K14_RS03400
• Protein ID: WP_000854753.1
• Coordinates: 712230..712604 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: I6K14_RS03405
• Protein ID: WP_001531802.1
• Coordinates: 712694..713062 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K14_RS03370 (707345)	707345..708493	-	1149	WP_000905914.1	capsule polysaccharide transporter	-
I6K14_RS03375 (708565)	708565..709548	-	984	WP_001335608.1	KpsF/GutQ family sugar-phosphate isomerase	-
I6K14_RS03380 (710358)	710358..710528	-	171	Protein_673	IS110 family transposase	-
I6K14_RS03385 (710870)	710870..711439	-	570	WP_001290247.1	DUF4942 domain-containing protein	-
I6K14_RS03390 (711536)	711536..711733	-	198	WP_000839281.1	DUF957 domain-containing protein	-
I6K14_RS03395 (711745)	711745..712233	-	489	WP_000777541.1	DUF5983 family protein	-
I6K14_RS03400 (712230)	712230..712604	-	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
I6K14_RS03405 (712694)	712694..713062	-	369	WP_001531802.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6K14_RS03410 (713225)	713225..713446	-	222	WP_000692329.1	DUF987 domain-containing protein	-
I6K14_RS03415 (713509)	713509..713985	-	477	WP_001186726.1	RadC family protein	-
I6K14_RS03420 (714001)	714001..714486	-	486	WP_001531954.1	antirestriction protein	-
I6K14_RS03425 (714541)	714541..715362	-	822	WP_001531953.1	DUF932 domain-containing protein	-
I6K14_RS24420 (715541)	715541..715630	-	90	WP_112031555.1	DUF905 family protein	-
I6K14_RS03435 (715773)	715773..716228	-	456	WP_000581502.1	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	710358..710462	104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T193460 WP_000854753.1 NZ_CP070170:c712604-712230 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T193460 NZ_CP092983:c406417-406232 [Leptospira interrogans]
ATGAAAGTCAAAGAGATCATCAAAATTTTAGAGGATGACGGTTGGTATTTAGTGGATCAAAGAGGAAGTCACAGACAGTT
TAAACATACCTTAAAATCTGGGAGAGTAACTGTAGCGGGAAAGTCGAGTATGGATTTACCTCCTGGAACCTTGAATAGTA
TCTTAAAACAATCCGGTCTAAAGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13422.27 Da
• Isoelectric Point: 6.3161

>AT193460 WP_001531802.1 NZ_CP070170:c713062-712694 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNSLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT193460 NZ_CP092983:c406215-406012 [Leptospira interrogans]
ATGGAATATCCAGTAATGATTGAAAAAAATTCGACCGGTTATGGAGCGTATGTTCCGGATTTACCTGGCTGTGTTGCAGT
TGGAGAAACGGAAGCGGAAGTAACTCGGTTAATCAAGGAAGCGATTGAGTTTCATTTAGAAGGTATGCGTAAAGATGGGG
AGCGGGTTCCTAATCCTTCCAAGGTAATGTTAATTGCGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0