Detailed information of TA system TA193457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 4326161..4326747
• Replicon: chromosome
• Accession: NZ_CP070169
• Organism: Klebsiella pneumoniae strain FDAARGOS_1320

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: I6K50_RS20640
• Protein ID: WP_002920800.1
• Coordinates: 4326379..4326747 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: I6K50_RS20635
• Protein ID: WP_004174006.1
• Coordinates: 4326161..4326382 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K50_RS20615	4322318..4323244	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
I6K50_RS20620	4323241..4324518	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
I6K50_RS20625	4324515..4325282	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
I6K50_RS20630	4325284..4325997	+	714	WP_009484078.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
I6K50_RS20635	4326161..4326382	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
I6K50_RS20640	4326379..4326747	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
I6K50_RS20645	4327020..4328336	+	1317	WP_002920796.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
I6K50_RS20650	4328443..4329330	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
I6K50_RS20655	4329327..4330172	+	846	WP_004174009.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
I6K50_RS20660	4330174..4331244	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4323241..4334772	11531

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T193457 WP_002920800.1 NZ_CP070169:4326379-4326747 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T193457 NZ_CP092981:c5394754-5394308 [Mycobacterium marinum]
ATGGCGAAACTTTCCGGGTCGATCGACGTGCCGCTGTCTCCGGACGAGGCATGGATGCACGCATCCGATCTCTCCCGCTT
CAAGGAGTGGCTGACGATCCACCGAGTGTGGCGCAGCACGTTGCCCGAAACCCTCGAAAAGGGTGCGGTCGTCGAATCAA
TCGTGCAGGTCAAGGGCATGCACAACCGCATCAAATGGACCATCGTGCGCTACCAGCCGCCGGAAGGCATGACCCTCAAC
GGTGACGGGGTCGGCGGCGTCAAGGTCAAGCTGATGGCCAAGGTTGCAGCCGGAGAAGAAGGCTCGGTCGTCAGCTTCGA
CGTGCACCTTGGTGGGCCGGCTCTGTTCGGTCCGATCGGCATGGTCGTCGCCGCGGCGCTGCGCACCGATATCAACGCAT
CGCTGCGGAACTTCGTCACCGTATTCGCCCCGTCGGCCGCCGGCTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT193457 WP_004174006.1 NZ_CP070169:4326161-4326382 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT193457 NZ_CP092981:c5394947-5394759 [Mycobacterium marinum]
ATGGGAATCCTGGACAAGGCGAAAGACCTGCTGGCGCAGAACGCCGACAAGGTCGAGACGGTGATCGACAAGGCCGGCGA
ATTCGTCGACGAGAAGACCGAGGGGAAGTACTCCGACACCATCCACAAGGTGCAAGAGGGGGCCAAGAAAGTCGCTGGTC
CCGGCGCCGCGGGCGAGCAGCAGAACTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A5E5YJY7