Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3923875..3924500 Replicon chromosome
Accession NZ_CP070169
Organism Klebsiella pneumoniae strain FDAARGOS_1320

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag I6K50_RS18780 Protein ID WP_002882817.1
Coordinates 3923875..3924258 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I6K50_RS18785 Protein ID WP_004150355.1
Coordinates 3924258..3924500 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K50_RS18765 3921241..3922143 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
I6K50_RS18770 3922140..3922775 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
I6K50_RS18775 3922772..3923701 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
I6K50_RS18780 3923875..3924258 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
I6K50_RS18785 3924258..3924500 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
I6K50_RS18790 3924705..3925622 + 918 WP_009484979.1 alpha/beta hydrolase -
I6K50_RS18795 3925636..3926577 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
I6K50_RS18800 3926622..3927059 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
I6K50_RS18805 3927056..3927916 - 861 WP_004146232.1 virulence factor BrkB family protein -
I6K50_RS18810 3927910..3928509 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T193456 WP_002882817.1 NZ_CP070169:c3924258-3923875 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T193456 NZ_CP092981:3461296-3461628 [Mycobacterium marinum]
GTGAATAGAGGGGAGATCTGGACCGTAGCGGGGGGCGTCTACGCGACCAAACCGCATCCCGCGGTGATTGTTCAGGATGA
TCTCTTTGATGCCACGAGTTCGGTGACGGTTGCTCCGATGTCCAGCACCCTGTTGGATGCGCCGTTGATGCGCATCCGAA
TAGCCGGCGGAGACGGCCGGTTATCCGGACTTGATCACAACAGTGACGTGATGATCGACAAGCTCACAACCGTGAAAAGG
TCGAACGTCCATGCCCGAGTCGGTCGGCTGACAGCGGAGCAAGTCGTCGAGGTCGAGCGAGTGATGATGGCATTCCTCGG
CCTTGCGCGATAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT193456 WP_004150355.1 NZ_CP070169:c3924500-3924258 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT193456 NZ_CP092981:3461093-3461299 [Mycobacterium marinum]
ATGGCGGTTAGGGACAGGGTCGGCGAGTACCGACGGCGGATGCGGGAGCGGGGCCTGCGGCCGCTGCAGGTCTGGGTGCC
TGACGTGCGCACTGAGAGTTTTGCTGCCGAGGCGCATCGTCAGGCTTCGTTGGTCGCACGAGCGGACGAACGCGGCGATG
ATCAGGACTTCATTGAGGCCATCTCGACGCCATGGGATGAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References