Detailed information of TA system TA193440

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4543246..4544080
• Replicon: chromosome
• Accession: NZ_CP070162
• Organism: Escherichia coli strain FDAARGOS_1283

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A8E0IWF2
• Locus tag: I6K13_RS22105
• Protein ID: WP_001521667.1
• Coordinates: 4543703..4544080 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: I6K13_RS22100
• Protein ID: WP_001285610.1
• Coordinates: 4543246..4543614 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K13_RS22065 (4539129)	4539129..4539809	+	681	WP_001282919.1	WYL domain-containing protein	-
I6K13_RS22070 (4539957)	4539957..4540634	+	678	WP_001521658.1	hypothetical protein	-
I6K13_RS22075 (4540640)	4540640..4540873	+	234	WP_001521659.1	DUF905 family protein	-
I6K13_RS22080 (4540972)	4540972..4541790	+	819	WP_021524626.1	DUF932 domain-containing protein	-
I6K13_RS22085 (4541882)	4541882..4542367	+	486	WP_021524627.1	antirestriction protein	-
I6K13_RS22090 (4542383)	4542383..4542859	+	477	WP_001186724.1	RadC family protein	-
I6K13_RS22095 (4542945)	4542945..4543166	+	222	WP_001521666.1	DUF987 domain-containing protein	-
I6K13_RS22100 (4543246)	4543246..4543614	+	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6K13_RS22105 (4543703)	4543703..4544080	+	378	WP_001521667.1	TA system toxin CbtA family protein	Toxin
I6K13_RS22110 (4544077)	4544077..4544226	+	150	Protein_4371	DUF5983 family protein	-
I6K13_RS22115 (4544302)	4544302..4544499	+	198	WP_086795284.1	DUF957 domain-containing protein	-
I6K13_RS22120 (4544584)	4544584..4545426	+	843	WP_113263653.1	DUF4942 domain-containing protein	-
I6K13_RS22125 (4546254)	4546254..4546517	+	264	WP_072277346.1	pas-pac-pac sensing his kinase	-
I6K13_RS22130 (4546695)	4546695..4547618	+	924	WP_001445306.1	carboxylate/amino acid/amine transporter	-
I6K13_RS22135 (4547622)	4547622..4548440	-	819	WP_000779409.1	lipoprotein NlpA	-
I6K13_RS22140 (4548662)	4548662..4548955	+	294	WP_001297978.1	protein YicS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4526277..4546517	20240

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14035.97 Da
• Isoelectric Point: 6.8607

>T193440 WP_001521667.1 NZ_CP070162:4543703-4544080 [Escherichia coli]
MKTLPVLPGQAASSRPSSVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTHSQLINSIDILRARRATGLMTRDNYRMVNDISQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T193440 NZ_CP092973:1855710-1855964 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT193440 WP_001285610.1 NZ_CP070162:4543246-4543614 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT193440 NZ_CP092973:1855462-1855713 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA