Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3595361..3596015 Replicon chromosome
Accession NZ_CP070162
Organism Escherichia coli strain FDAARGOS_1283

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag I6K13_RS17470 Protein ID WP_000244781.1
Coordinates 3595361..3595768 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I6K13_RS17475 Protein ID WP_000354046.1
Coordinates 3595749..3596015 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K13_RS17450 (3591318) 3591318..3593051 - 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -
I6K13_RS17455 (3593057) 3593057..3593767 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6K13_RS17460 (3593792) 3593792..3594688 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
I6K13_RS17465 (3594800) 3594800..3595321 + 522 WP_001055874.1 flavodoxin FldB -
I6K13_RS17470 (3595361) 3595361..3595768 - 408 WP_000244781.1 protein YgfX Toxin
I6K13_RS17475 (3595749) 3595749..3596015 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I6K13_RS17480 (3596258) 3596258..3597238 + 981 WP_000886080.1 tRNA-modifying protein YgfZ -
I6K13_RS17485 (3597315) 3597315..3597974 - 660 WP_000250274.1 hemolysin III family protein -
I6K13_RS17490 (3598138) 3598138..3598449 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
I6K13_RS17495 (3598494) 3598494..3599927 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T193439 WP_000244781.1 NZ_CP070162:c3595768-3595361 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T193439 NZ_CP092973:1855462-1855713 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT193439 WP_000354046.1 NZ_CP070162:c3596015-3595749 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT193439 NZ_CP092973:1855710-1855964 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References