Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 947294..947912 Replicon chromosome
Accession NZ_CP070162
Organism Escherichia coli strain FDAARGOS_1283

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6K13_RS04435 Protein ID WP_001291435.1
Coordinates 947294..947512 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6K13_RS04440 Protein ID WP_000344800.1
Coordinates 947538..947912 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K13_RS04400 (942582) 942582..943154 + 573 WP_000779831.1 YbaY family lipoprotein -
I6K13_RS04405 (943185) 943185..943496 - 312 WP_000409908.1 MGMT family protein -
I6K13_RS04415 (943875) 943875..944228 + 354 WP_000878140.1 DUF1428 domain-containing protein -
I6K13_RS04420 (944270) 944270..945820 - 1551 WP_021523794.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6K13_RS04425 (945984) 945984..946454 - 471 WP_000136192.1 YlaC family protein -
I6K13_RS04430 (946570) 946570..947121 - 552 WP_000102539.1 maltose O-acetyltransferase -
I6K13_RS04435 (947294) 947294..947512 - 219 WP_001291435.1 HHA domain-containing protein Toxin
I6K13_RS04440 (947538) 947538..947912 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6K13_RS04445 (948457) 948457..951606 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
I6K13_RS04450 (951629) 951629..952822 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T193419 WP_001291435.1 NZ_CP070162:c947512-947294 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T193419 NZ_CP092968:2861909-2862316 [Methylocystis parvus OBBP]
ATGTTCTGTCTGGATACGAATGTGGTCATTTTCGCCCTCAACGGCCGGCGCGCGGGCTATGCGGAGCGGCTGGCGGAAGA
GATCCGGGCCGGGACGAAGCTGATCGTTCCGTCTGTCGTTTTGTTCGAGCTCTCTTACGGGATCGCAAAAAGTCGGCAGC
CCGAAAAAGCCCGAGCGCTTCTCGTTGAATTTCTCTCCGCCGGGTTTGAACAGCCGGCGTTCGATGCCGACGATGCGCAC
GAAGCCGGCGACATTCGCGCCCATTTGGAAGCGCGCGGCGCGCCGATTGGCCCCTTCGACGTTCTCATCGCCGCTCAGGC
GCGGCGTCGCGGCGCCGTGCTCGTCACAAATAACCGCCGCGAATTTGACCGCGTGCCGGGCTTGATGGCGACAGACTGGG
CGGCTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT193419 WP_000344800.1 NZ_CP070162:c947912-947538 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT193419 NZ_CP092968:2861646-2861900 [Methylocystis parvus OBBP]
ATGGCGACGGCGAAACTGTTCCAGCACGGTCGCAGCCAGGCGGTTCGCTTGCCGAAGGAATTCCGGCTGCCAGGGACGGA
GGTCCGGGTCAGCAAGGTGGGCGACAAGGTCATTCTCGAGCCGCTGGAATCCGAGCCTTTCGATGTCGACGCCTGGCGAT
TGCGGTTGGACGCTCTCGGCGCGAAGGATTTCTTGCCGGAGGGCATTCCCGACGATCAGCCGCTTCGGCCCGACGAATCT
ATTTCATTCGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References