Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 515964..516514 Replicon chromosome
Accession NZ_CP070162
Organism Escherichia coli strain FDAARGOS_1283

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag I6K13_RS02430 Protein ID WP_001160966.1
Coordinates 516200..516514 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag I6K13_RS02425 Protein ID WP_000125566.1
Coordinates 515964..516197 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K13_RS02400 (511037) 511037..511324 + 288 WP_000203741.1 ferredoxin-like protein FixX -
I6K13_RS02405 (511383) 511383..512714 + 1332 WP_001183207.1 MFS transporter -
I6K13_RS02410 (512822) 512822..513352 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
I6K13_RS02415 (513345) 513345..515207 + 1863 WP_113263567.1 glutathione-regulated potassium-efflux system protein KefC -
I6K13_RS02420 (515399) 515399..515878 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
I6K13_RS02425 (515964) 515964..516197 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
I6K13_RS02430 (516200) 516200..516514 + 315 WP_001160966.1 CcdB family protein Toxin
I6K13_RS02435 (516511) 516511..517359 - 849 WP_001765384.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
I6K13_RS02440 (517366) 517366..517743 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
I6K13_RS02445 (517746) 517746..518567 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
I6K13_RS02450 (518564) 518564..519553 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
I6K13_RS02455 (519553) 519553..520839 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T193417 WP_001160966.1 NZ_CP070162:516200-516514 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T193417 NZ_CP092968:c1218012-1217758 [Methylocystis parvus OBBP]
ATGCGCACCACTTCTTACAGCGATCTCCGCAAGAACCTCGCCGCCACGCTCGACCGCGTGACCGAAGATCATGAGCCGGT
AGTCATCACGCGCGATCGCGGCAAGCCCGCCGCCGTTCTCATTTCGCTCGAGGACTTCGCGTCCTATGAGGAGACCGCGC
ATCTTTTGAAGAATCCGCGCAACGCCGAGCGCCTTCTCGCTGCGATCGGTGAACTGGACGCGGGCAAGGGCGTGGAGCGC
AAGCTCTCGGAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT193417 WP_000125566.1 NZ_CP070162:515964-516197 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT193417 NZ_CP092968:c1217761-1217507 [Methylocystis parvus OBBP]
ATGAAGCTCGTCTTTGCGGAACAGGCCTGGGAGGATTACCTCTACTGGCAGTCGCAGGACGCGAAGGTTCTGGAGCGCAT
TAATTCACTCATCAAGGAATGTGCGCGCACGCCTTTTTCAGGGACGGGAAAGCCCGAGCCGCTACGGGGCGACTTGCGCG
GCTGGTGGTCGCGACGGATCACGCTGGAGCATCGGCTGGTGTATCGCGTTGAAGGCGAGAGCCTGCTGATTGCACAGTGC
AGGTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References