Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 27246..27743 Replicon chromosome
Accession NZ_CP070162
Organism Escherichia coli strain FDAARGOS_1283

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I6K13_RS00130 Protein ID WP_001173343.1
Coordinates 27570..27743 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag I6K13_RS00125 Protein ID WP_001312331.1
Coordinates 27246..27542 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K13_RS00105 (23977) 23977..24696 - 720 WP_000611282.1 two-component system response regulator DcuR -
I6K13_RS00110 (24693) 24693..26324 - 1632 WP_001216466.1 sensor histidine kinase -
I6K13_RS00115 (26505) 26505..26735 + 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
I6K13_RS00120 (26747) 26747..27019 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
I6K13_RS00125 (27246) 27246..27542 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6K13_RS00130 (27570) 27570..27743 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I6K13_RS00135 (27862) 27862..29379 - 1518 WP_000003805.1 lysine--tRNA ligase -
I6K13_RS00140 (29616) 29616..31073 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T193414 WP_001173343.1 NZ_CP070162:27570-27743 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T193414 NZ_CP092923:781281-781565 [Pseudomonas syringae pv. tagetis]
ATGAAAGAACAAATTTTCGATTACGACCCAGCAGCAGCCCTTGACGGGCCTGAGGCAATTGCGATTTTTATCTCTGACGC
ATTTGAGACGGGGGATGCGGAGTACATCGCGAAGGCTATGGGGGTTGTCGCAAGGGCCAAAGGGATGACCGAGCTTGCGC
GTGAGACAGGTCTTTCTCGAGAGCAGCTTTACAGATCGTTCAGTAGCAAGGGCAATCCAACCTTAAAAACCATGTTGACG
GTAATGAGAGCCCTGGGCGTGGACATGACAGCTCGAATGCACTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT193414 WP_001312331.1 NZ_CP070162:27246-27542 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT193414 NZ_CP092923:780985-781284 [Pseudomonas syringae pv. tagetis]
ATGACCACGATAAAACAGACCTCAACCTATATGGCTTGGGAACGCAGGCTCAAAGACCAAAAGGCCAAAGCTGCAATTGC
CGCTCGTATTTTTAGAGTTGCAAATGGCCTGATGGGTGATGTCTCGCCAGTAGGGCAGGGCGTGAGTGAGCTACGGATAC
ACGTTGGCCCTGGTTACCGTGTTTATTTCCAGCAGCGAGGCGATGAACTGATCTTGCTGCTGTGCGGTGGTGACAAGAGC
AGCCAATCCCGAGATATCGAAACAGCCAAGAAACTGGCTGATCAATGGAGGCAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References