Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4505036..4505654 Replicon chromosome
Accession NZ_CP070157
Organism Escherichia coli strain FDAARGOS_1276

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6K06_RS21430 Protein ID WP_001291435.1
Coordinates 4505036..4505254 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6K06_RS21435 Protein ID WP_000344800.1
Coordinates 4505280..4505654 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K06_RS21395 (4500325) 4500325..4500897 + 573 WP_000779831.1 YbaY family lipoprotein -
I6K06_RS21400 (4500928) 4500928..4501239 - 312 WP_000409911.1 MGMT family protein -
I6K06_RS21410 (4501618) 4501618..4501971 + 354 WP_001550741.1 DUF1428 family protein -
I6K06_RS21415 (4502013) 4502013..4503563 - 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6K06_RS21420 (4503727) 4503727..4504197 - 471 WP_000136192.1 YlaC family protein -
I6K06_RS21425 (4504313) 4504313..4504864 - 552 WP_000102564.1 maltose O-acetyltransferase -
I6K06_RS21430 (4505036) 4505036..4505254 - 219 WP_001291435.1 HHA domain-containing protein Toxin
I6K06_RS21435 (4505280) 4505280..4505654 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6K06_RS21440 (4506200) 4506200..4509349 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
I6K06_RS21445 (4509372) 4509372..4510565 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T193391 WP_001291435.1 NZ_CP070157:c4505254-4505036 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T193391 NZ_CP092908:c61984-61643 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGAAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACATTGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCAACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGACTTTGCAGAAAGAAAGTTAAGCCAAATAGAATATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT193391 WP_000344800.1 NZ_CP070157:c4505654-4505280 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT193391 NZ_CP092908:c62214-61984 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTAATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGACCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References