Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2744764..2745261 Replicon chromosome
Accession NZ_CP070157
Organism Escherichia coli strain FDAARGOS_1276

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I6K06_RS13200 Protein ID WP_001173343.1
Coordinates 2744764..2744937 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag I6K06_RS13205 Protein ID WP_000398619.1
Coordinates 2744965..2745261 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K06_RS13190 (2741434) 2741434..2742891 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
I6K06_RS13195 (2743128) 2743128..2744645 + 1518 WP_001550472.1 lysine--tRNA ligase -
I6K06_RS13200 (2744764) 2744764..2744937 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I6K06_RS13205 (2744965) 2744965..2745261 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6K06_RS13210 (2745490) 2745490..2745762 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
I6K06_RS13215 (2745774) 2745774..2746004 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
I6K06_RS13220 (2746184) 2746184..2747815 + 1632 WP_001216477.1 sensor histidine kinase -
I6K06_RS13225 (2747812) 2747812..2748531 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T193385 WP_001173343.1 NZ_CP070157:c2744937-2744764 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T193385 NZ_CP092907:c2568463-2568362 [Enterococcus faecalis]
TTGTCAATCGAAGCGACATTGGAATTGATGATTAGTTTTGCAACCCTTGTTGCGTTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACAATAAAAAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT193385 WP_000398619.1 NZ_CP070157:c2745261-2744965 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT193385 NZ_CP092907:2568240-2568425 [Enterococcus faecalis]
AAAAGAGAGGTATGCGGCTACATACCTCTCCTTTATACCAGCGCCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGTTT
TTATTACTGTTTAACCGTTCGGCCTGTCAAGCTTATGAACGGTTATTTTTTATTGTTTTTCGTTGCTTCAAGGATACCGA
AAATCAGTAACGCAACAAGGGTTGCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References