Detailed information of TA system TA193369

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 486000..486638
• Replicon: chromosome
• Accession: NZ_CP070157
• Organism: Escherichia coli strain FDAARGOS_1276

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: I6K06_RS02350
• Protein ID: WP_000813795.1
• Coordinates: 486000..486176 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: I6K06_RS02355
• Protein ID: WP_076797675.1
• Coordinates: 486222..486638 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K06_RS02330 (481622)	481622..482794	-	1173	WP_001551088.1	BenE family transporter YdcO	-
I6K06_RS02335 (482886)	482886..483422	+	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
I6K06_RS02340 (483495)	483495..485456	+	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
I6K06_RS02345 (485548)	485548..485778	-	231	WP_023910283.1	YncJ family protein	-
I6K06_RS02350 (486000)	486000..486176	+	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
I6K06_RS02355 (486222)	486222..486638	+	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
I6K06_RS02360 (486717)	486717..488123	+	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
I6K06_RS02365 (488368)	488368..489513	+	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
I6K06_RS02370 (489531)	489531..490544	+	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
I6K06_RS02375 (490545)	490545..491486	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T193369 WP_000813795.1 NZ_CP070157:486000-486176 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T193369 NZ_CP092901:833552-833962 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT193369 WP_076797675.1 NZ_CP070157:486222-486638 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT193369 NZ_CP092901:833305-833571 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1