Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 5024115..5024753 Replicon chromosome
Accession NZ_CP070152
Organism Escherichia coli strain FDAARGOS_1285

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag I6K15_RS24460 Protein ID WP_000813794.1
Coordinates 5024115..5024291 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag I6K15_RS24465 Protein ID WP_001270286.1
Coordinates 5024337..5024753 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K15_RS24440 (5019737) 5019737..5020909 - 1173 WP_022645663.1 BenE family transporter YdcO -
I6K15_RS24445 (5021001) 5021001..5021537 + 537 WP_000429148.1 DNA-binding transcriptional regulator SutR -
I6K15_RS24450 (5021610) 5021610..5023571 + 1962 WP_077737811.1 23S rRNA 5-hydroxycytidine C2501 synthase -
I6K15_RS24455 (5023663) 5023663..5023893 - 231 WP_000491567.1 DUF2554 family protein -
I6K15_RS24460 (5024115) 5024115..5024291 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
I6K15_RS24465 (5024337) 5024337..5024753 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
I6K15_RS24470 (5024832) 5024832..5026238 + 1407 WP_022645665.1 PLP-dependent aminotransferase family protein -
I6K15_RS24475 (5026483) 5026483..5027628 + 1146 WP_000047466.1 ABC transporter substrate-binding protein -
I6K15_RS24480 (5027646) 5027646..5028659 + 1014 WP_022645666.1 ABC transporter ATP-binding protein -
I6K15_RS24485 (5028660) 5028660..5029601 + 942 WP_022645667.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T193367 WP_000813794.1 NZ_CP070152:5024115-5024291 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T193367 NZ_CP092901:325284-325652 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT193367 WP_001270286.1 NZ_CP070152:5024337-5024753 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT193367 NZ_CP092901:325066-325287 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References