Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4620339..4621123 Replicon chromosome
Accession NZ_CP070152
Organism Escherichia coli strain FDAARGOS_1285

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag I6K15_RS22460 Protein ID WP_000613626.1
Coordinates 4620339..4620833 (-) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A0A1AEM7
Locus tag I6K15_RS22465 Protein ID WP_024946541.1
Coordinates 4620830..4621123 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K15_RS22440 (4615532) 4615532..4616629 + 1098 WP_021580666.1 flagellar basal body P-ring protein FlgI -
I6K15_RS22445 (4616629) 4616629..4617570 + 942 WP_001317765.1 flagellar assembly peptidoglycan hydrolase FlgJ -
I6K15_RS22450 (4617636) 4617636..4619279 + 1644 WP_022645523.1 flagellar hook-associated protein FlgK -
I6K15_RS22455 (4619291) 4619291..4620244 + 954 WP_022645524.1 flagellar hook-associated protein FlgL -
I6K15_RS22460 (4620339) 4620339..4620833 - 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
I6K15_RS22465 (4620830) 4620830..4621123 - 294 WP_024946541.1 DUF1778 domain-containing protein Antitoxin
I6K15_RS22470 (4621257) 4621257..4624430 - 3174 WP_063814920.1 ribonuclease E -
I6K15_RS22475 (4625003) 4625003..4625962 + 960 WP_022645527.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T193358 WP_000613626.1 NZ_CP070152:c4620833-4620339 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T193358 NZ_CP092891:c3597850-3597449 [Microbacterium oxydans]
GTGATCCTCGTCGACACCTCCGTCTGGATCGCCTATCTCCGCGGGACCGACTCACCGGCCGCCGAGCAGCTGAACGGGCT
GATCGCCGAGGGGGCAGACCTCGCGATCACGGAACCGGTGATCATGGAGCTCCTGGCGGGCGCCGCCAGGCCGCGGGAGC
GGGCGAGGGTGGAGGTGTTGACGAACGGGCTGCCGCTGATCGGGATCGACCCGGTGGGCGACTTCCGGGACGCCGCCGAC
CTGTACCGTGCGTCCGCGCTCAACGGGCACCCGATCCGGAGCATGACCGACTGCCTGATCGCGGCCGTGGCGATCCGCAG
CGACGTGGCGCTGCTGCACGGCGATCGTGACTTCGAGTTCCTCGCGGAGGTCAGCCCGCTTCGGCTCTGTCCGACCGAGT
AG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11039.60 Da        Isoelectric Point: 10.5423

>AT193358 WP_024946541.1 NZ_CP070152:c4621123-4620830 [Escherichia coli]
MAAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRSEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT193358 NZ_CP092891:c3598065-3597847 [Microbacterium oxydans]
ATGGCGCGCACGAACATCGACATCGACGAAGACCTGGTGCGTGAGGTGATGCGCAGGTACAGCCTCTCCACCAAGAAGGA
GGCCGTCGATCTCGCGCTCCGCCGCCTCGTCGGAGTGCCCCTGACGAAGGAGTTCCTCCTCGGCCTGCGGGGGACGGGCT
GGAGCGGCGATCTCGACGCGATGCGCGACGACGCACCGGCGTCCTGGGGCGACGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB A0A0A1AEM7

References