Detailed information of TA system TA193357

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 4362980..4363685
• Replicon: chromosome
• Accession: NZ_CP070152
• Organism: Escherichia coli strain FDAARGOS_1285

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: I6K15_RS21255
• Protein ID: WP_000539521.1
• Coordinates: 4363299..4363685 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: I6K15_RS21250
• Protein ID: WP_001280945.1
• Coordinates: 4362980..4363309 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K15_RS21230 (4358450)	4358450..4360798	+	2349	WP_077737787.1	EAL domain-containing protein	-
I6K15_RS21235 (4360806)	4360806..4361120	+	315	Protein_4043	hypothetical protein	-
I6K15_RS21240 (4361176)	4361176..4361873	+	698	WP_094096600.1	IS1-like element IS1A family transposase	-
I6K15_RS21245 (4361888)	4361888..4362910	+	1023	Protein_4045	diguanylate cyclase	-
I6K15_RS21250 (4362980)	4362980..4363309	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
I6K15_RS21255 (4363299)	4363299..4363685	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K15_RS21260 (4363911)	4363911..4365236	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
I6K15_RS21265 (4365449)	4365449..4365832	+	384	WP_000497137.1	biofilm formation regulator BssR	-
I6K15_RS21270 (4365943)	4365943..4367058	+	1116	WP_000554964.1	aldose sugar dehydrogenase YliI	-
I6K15_RS21275 (4367055)	4367055..4367681	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T193357 WP_000539521.1 NZ_CP070152:c4363685-4363299 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T193357 NZ_CP092888:c1988119-1987892 [Bifidobacterium sp. FKU]
ATGAAGAAGCTGGATCGTAATGTTGCCAAGAGAATCATCGCAAAGCTTAGGGAAATTTCACAGTTGGAAGATCCGAGAAG
CACCGGCAAGGCTCTTGCCGGTAATCTGGCAGGGCTGTGGCGTTACCGTGTCGGGGACTATCGTATCGTGTGCGATATAG
AGGATGAGGTTCTGCTGATTCTTGTGATTGATGTCGCGCACAGGAGCAAGGTGTACAAGCGTTGCTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT193357 WP_001280945.1 NZ_CP070152:c4363309-4362980 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT193357 NZ_CP092888:c1988381-1988145 [Bifidobacterium sp. FKU]
ATGGCCACGGTTACTGCAGCATTAAGGATGCCTGTCGAATTGGCGGCTCGTTACGATTGTCTGGCGAAAGCGACTGGTCG
TACGAAGACGTTTTACATGAACGAAGCCCTTACCGAATCGATCGATAGGTTTGAGTATGAATACGGCATTATGAAAAAGG
TCGAGGATTGGCGTGCCGGCAGACTCGAAACCGTGACGCTCGACGAGCTGGAGGAAAGCCTTGGCTTGGAGGATTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6