Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3953543..3954161 Replicon chromosome
Accession NZ_CP070152
Organism Escherichia coli strain FDAARGOS_1285

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6K15_RS19420 Protein ID WP_001291435.1
Coordinates 3953543..3953761 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6K15_RS19425 Protein ID WP_000344800.1
Coordinates 3953787..3954161 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K15_RS19385 (3948832) 3948832..3949404 + 573 WP_000779831.1 YbaY family lipoprotein -
I6K15_RS19390 (3949435) 3949435..3949746 - 312 WP_000409911.1 MGMT family protein -
I6K15_RS19400 (3950125) 3950125..3950478 + 354 WP_000878140.1 DUF1428 domain-containing protein -
I6K15_RS19405 (3950520) 3950520..3952070 - 1551 WP_022645279.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6K15_RS19410 (3952234) 3952234..3952704 - 471 WP_022645280.1 YlaC family protein -
I6K15_RS19415 (3952820) 3952820..3953371 - 552 WP_000102574.1 maltose O-acetyltransferase -
I6K15_RS19420 (3953543) 3953543..3953761 - 219 WP_001291435.1 HHA domain-containing protein Toxin
I6K15_RS19425 (3953787) 3953787..3954161 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6K15_RS19430 (3954707) 3954707..3957856 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
I6K15_RS19435 (3957879) 3957879..3959072 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T193355 WP_001291435.1 NZ_CP070152:c3953761-3953543 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T193355 NZ_CP092888:1533802-1534158 [Bifidobacterium sp. FKU]
ATGATGAAGACTGATCCGCGCCAGTTCGAGATATGGTGGGTGCCGTTCGCTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAATCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCGCCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT193355 WP_000344800.1 NZ_CP070152:c3954161-3953787 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT193355 NZ_CP092888:1533519-1533815 [Bifidobacterium sp. FKU]
ATGATGGCTATCAAGGAGAAGACCACAATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCAT
GGGGTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCG
TCCGCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCC
CAGATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References