Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3533799..3534349 Replicon chromosome
Accession NZ_CP070152
Organism Escherichia coli strain FDAARGOS_1285

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag I6K15_RS17455 Protein ID WP_001160966.1
Coordinates 3534035..3534349 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag I6K15_RS17450 Protein ID WP_000125568.1
Coordinates 3533799..3534032 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K15_RS17425 (3528872) 3528872..3529159 + 288 WP_000203741.1 ferredoxin-like protein FixX -
I6K15_RS17430 (3529217) 3529217..3530548 + 1332 WP_001183201.1 MFS transporter -
I6K15_RS17435 (3530656) 3530656..3531186 + 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
I6K15_RS17440 (3531179) 3531179..3533042 + 1864 Protein_3306 glutathione-regulated potassium-efflux system protein KefC -
I6K15_RS17445 (3533234) 3533234..3533713 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
I6K15_RS17450 (3533799) 3533799..3534032 + 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
I6K15_RS17455 (3534035) 3534035..3534349 + 315 WP_001160966.1 CcdB family protein Toxin
I6K15_RS17460 (3534346) 3534346..3535194 - 849 WP_000257195.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
I6K15_RS17465 (3535201) 3535201..3535578 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
I6K15_RS17470 (3535581) 3535581..3536402 - 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
I6K15_RS17475 (3536399) 3536399..3537388 - 990 WP_000241224.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
I6K15_RS17480 (3537388) 3537388..3538674 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T193353 WP_001160966.1 NZ_CP070152:3534035-3534349 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T193353 NZ_CP092888:c1045420-1045118 [Bifidobacterium sp. FKU]
ATCAAGCAATCCGCCGAATACCGCAAGTGGTTCAAGAAACTCAGGGACCACAAAGCGAAGGCCGCCATCCAAGCCCGGCT
CGATGCCTGCAAGCTCGCAGGCAGGCCATTCGGCGACATCAAACCCGTGGGAGGCCCGGTCAGCGAGATGCGGTTCCACA
CCGGGGCCGGATACCGCGTCTACTTCGCCATGCAGGGCAACGTGCTCATGCTGCTGCTCGCAGGCGGCGACAAAAGCACC
CAGCAGACCGACATCAGACAAGCCCACGACATCCTCAACGACTACAAGGAACAACGACAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT193353 WP_000125568.1 NZ_CP070152:3533799-3534032 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT193353 NZ_CP092888:c1045121-1044837 [Bifidobacterium sp. FKU]
ATGAGCACCGAATTCACCGACTACGACACCAGCGAATACCTCGAAAACGAACAGGACATCATCGCCTACCTCAACGCCAT
AGCCGAATATGACGACCCCGCACTCATGCAGGCCGCGCTCGGCAACGTCGCCAAGGCCCGGGGCATGACCCAGATCGCCA
AGGACGCGGGCGTGGGGCGCGAAAGCCTCTACAAGAGCCTCAGCAAGGACGGCAACCCCAGCTTCCAGACCATCGCCAAG
GTAATCCACGCCCTCGGCGGACGCCTCACCATCCAAGCCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References