Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3121911..3122408 Replicon chromosome
Accession NZ_CP070152
Organism Escherichia coli strain FDAARGOS_1285

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I6K15_RS15550 Protein ID WP_001173343.1
Coordinates 3122235..3122408 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag I6K15_RS15545 Protein ID WP_000398619.1
Coordinates 3121911..3122207 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K15_RS15525 (3118641) 3118641..3119360 - 720 WP_000611288.1 two-component system response regulator DcuR -
I6K15_RS15530 (3119357) 3119357..3120988 - 1632 WP_022646455.1 sensor histidine kinase -
I6K15_RS15535 (3121169) 3121169..3121399 + 231 WP_001683478.1 4Fe-4S mono-cluster protein YjdI -
I6K15_RS15540 (3121411) 3121411..3121683 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
I6K15_RS15545 (3121911) 3121911..3122207 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6K15_RS15550 (3122235) 3122235..3122408 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I6K15_RS15555 (3122527) 3122527..3124044 - 1518 WP_204628311.1 lysine--tRNA ligase -
I6K15_RS15560 (3124281) 3124281..3125738 - 1458 WP_022646457.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T193350 WP_001173343.1 NZ_CP070152:3122235-3122408 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T193350 NZ_CP092862:226269-226556 [Salmonella enterica]
ATGACTTACATAGTCAGATTCAGGGAAGATGCACTGAAAGAATGGCAAAAACTGGATAAGGCTATTCAGCGGCAGTTTGC
TAAAAAGCTAAAGAAGTGCTGTGAAAATCCACATATCCCATCAGCAAAACTGCGCGGGATAAAGGATTGCTACAAAATTA
AACTTCGTGCGTCTGGCTTCCGCCTGGTTTATCAGGTGATTGACGCTCAATTAATTATCGCTGTTGTGGCTGTAGGCAAA
CGTGAACGTAGTGACGTTTATAATCTTGCCAGCGAACGAATGAGGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT193350 WP_000398619.1 NZ_CP070152:3121911-3122207 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT193350 NZ_CP092862:226021-226272 [Salmonella enterica]
ATGCCCAACATTATTCTGAGTGACACTAGCGCCAGCGTCAGCGAACTGAAAAAAAACCCGATGGCGATAGTCAGTGCCGG
TGATGGTTACCCGGTCGCGATTCTGAACCGCAACCAGCCCGCCTTTTACTGTGTCCCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAACTGGTTAAGCTGATCGACGAACGCAGCAACCAGAAGCTGCACGACGTGGATCTGGAT
AGCTACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References