Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3623585..3624168 Replicon chromosome
Accession NZ_CP070148
Organism Escherichia coli strain FDAARGOS_1297

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag I6K27_RS17510 Protein ID WP_000254750.1
Coordinates 3623585..3623920 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag I6K27_RS17515 Protein ID WP_000581937.1
Coordinates 3623920..3624168 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K27_RS17495 (3619471) 3619471..3620769 - 1299 WP_000036723.1 phosphopyruvate hydratase -
I6K27_RS17500 (3620857) 3620857..3622494 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
I6K27_RS17505 (3622722) 3622722..3623513 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
I6K27_RS17510 (3623585) 3623585..3623920 - 336 WP_000254750.1 endoribonuclease MazF Toxin
I6K27_RS17515 (3623920) 3623920..3624168 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
I6K27_RS17520 (3624246) 3624246..3626480 - 2235 WP_000226815.1 GTP pyrophosphokinase -
I6K27_RS17525 (3626528) 3626528..3627829 - 1302 WP_000046816.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T193331 WP_000254750.1 NZ_CP070148:c3623920-3623585 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T193331 NZ_CP092860:c3042966-3042863 [Citrobacter braakii]
GGCAGGGTAACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT193331 WP_000581937.1 NZ_CP070148:c3624168-3623920 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT193331 NZ_CP092860:3042861-3043001 [Citrobacter braakii]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAGTTACCCTGCCATTTAAGAATAGATGACAGCGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References