Detailed information of TA system TA193320

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2218966..2220554
• Replicon: chromosome
• Accession: NZ_CP070148
• Organism: Escherichia coli strain FDAARGOS_1297

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B7MMY1
• Locus tag: I6K27_RS10745
• Protein ID: WP_001125475.1
• Coordinates: 2218966..2220288 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: I6K27_RS10750
• Protein ID: WP_001332145.1
• Coordinates: 2220288..2220554 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K27_RS10710 (2214522)	2214522..2214755	-	234	WP_000473544.1	hypothetical protein	-
I6K27_RS10715 (2214790)	2214790..2215041	-	252	WP_000381239.1	membrane protein	-
I6K27_RS10720 (2215044)	2215044..2215361	-	318	WP_000073835.1	DNA-binding protein	-
I6K27_RS10725 (2215373)	2215373..2216425	-	1053	WP_000568110.1	phage/plasmid replication protein, II/X family	-
I6K27_RS10730 (2216597)	2216597..2217043	+	447	WP_000527682.1	helix-turn-helix transcriptional regulator	-
I6K27_RS10735 (2217143)	2217143..2217565	+	423	WP_000089310.1	hypothetical protein	-
I6K27_RS10740 (2217673)	2217673..2218782	+	1110	WP_001555908.1	hypothetical protein	-
I6K27_RS10745 (2218966)	2218966..2220288	-	1323	WP_001125475.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
I6K27_RS10750 (2220288)	2220288..2220554	-	267	WP_001332145.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
I6K27_RS10755 (2220862)	2220862..2221620	+	759	WP_001286588.1	trans-aconitate 2-methyltransferase	-
I6K27_RS10760 (2221624)	2221624..2222538	-	915	WP_001306069.1	bestrophin family protein	-
I6K27_RS10765 (2222735)	2222735..2224186	-	1452	WP_000854637.1	tagaturonate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimA	2187159..2220554	33395

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49426.24 Da
• Isoelectric Point: 8.9380

>T193320 WP_001125475.1 NZ_CP070148:c2220288-2218966 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRRNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVMCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T193320 NZ_CP092857:c275133-274864 [Staphylococcus capitis]
ATGAGTAAGTTTAGTGTAACTTTTACAGCTCAAGCATTTAAAGATTATCAATATTGGCAACAACACAATCAAAATTATTT
TAAAAAAGTGAATGCTTTACTTAAAAGTATTGCAAGAGATGGTTGTGTCGATGGTATTGGAAAACCAGAAGCATTAAAAG
GAAACTACGAAGGCTATTTCAGTAGAAGAGTTTCAATAGAACACCGATTAATATACAAAATTAAGGATGATTCAATATTC
GTAGTGTCTTGCAGATTTCATTATCAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.44 Da
• Isoelectric Point: 6.2267

>AT193320 WP_001332145.1 NZ_CP070148:c2220554-2220288 [Escherichia coli]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPNNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT193320 NZ_CP092857:c275383-275126 [Staphylococcus capitis]
ATGCCTTCAGTTAATTATTCAAACGCTAGACAGAATTTCAAAGAACTTATTACTAAAGTGAACGAGGATAGTGTGGCTTA
CACTATTACGACAAAGGAAAATGAAAATGCAGTGATTTTAGCTGAAAAGGATTATAATGCGATGTTGGAAACGATGTACC
TTCAATCCAATCTCGCTAATGTGTCTCACTTAAATCAATCAATTATGGAATTGAATCATAACCAAACAATTACAATAGAG
ATTGATGATAATGAGTAA

Structures

Toxin

• AlphaFold DB: A0A0E2L5M8