Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 150971..151468 Replicon chromosome
Accession NZ_CP070148
Organism Escherichia coli strain FDAARGOS_1297

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I6K27_RS00690 Protein ID WP_001173343.1
Coordinates 151295..151468 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag I6K27_RS00685 Protein ID WP_001312331.1
Coordinates 150971..151267 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K27_RS00665 (147700) 147700..148419 - 720 WP_000611291.1 two-component system response regulator DcuR -
I6K27_RS00670 (148416) 148416..150047 - 1632 WP_001216482.1 sensor histidine kinase -
I6K27_RS00675 (150228) 150228..150458 + 231 WP_000373293.1 4Fe-4S mono-cluster protein YjdI -
I6K27_RS00680 (150470) 150470..150742 + 273 WP_000405646.1 GNAT family N-acetyltransferase -
I6K27_RS00685 (150971) 150971..151267 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6K27_RS00690 (151295) 151295..151468 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I6K27_RS00695 (151587) 151587..153104 - 1518 WP_000003806.1 lysine--tRNA ligase -
I6K27_RS00700 (153341) 153341..154798 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T193307 WP_001173343.1 NZ_CP070148:151295-151468 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T193307 NZ_CP092848:4411435-4411860 [Methylobacterium organophilum]
ATGATCGTCCTCGACACCAACGTCATTTCCGAATTGTGGAGGGTGGTGCCCGATCCCGCCGTGCGGGCTTGGATCGATGC
CCAGGTCGTCGAGACCCTCTACCTCTCGGCCGTCACCGTTGCCGAACTCCGTCTCGGCATCGCGGTGATGTCGGCCGGCA
AGCGGCAGAGGACCTATCGGGATCGGCTGGAGCGGGAGGTGCTGCCTGCCTTTGCCGGTCGCGTGCTTCCCTTCGACCTC
GATGCCGCGCGGCATTACGCGGACTTCATGGCACAGGCGAAGACGGCGGGACGGGCAATTGCCCAGGCAGACGGCCACAT
CGCGGCCATCGCCGCGGCACGGGGCTTCATCGTCGCCAGCCGCGACACGAGCCCGTTCGAGGCCGCCGGGGTGAGAGTCG
TCGATCCCTGGAAAGCCGCGGCATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT193307 WP_001312331.1 NZ_CP070148:150971-151267 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT193307 NZ_CP092848:4411181-4411438 [Methylobacterium organophilum]
ATGCGCTCCGTCACCATTCGCAATATTCCCGAGGACGTCCACCGTGCGCTGCGGGTCCGGGCCGCGCGGCACGGGCGCAG
CACCGAGGCCGAGATCCGGGACATCCTCGAGCAGGCGGCCAAGCCCGAAGGCCGCCTGAAACTCGGTTCGCTGCTGACCT
CCATCGCCCGCGAGGCCGGACCCTTGTCGGAGGAAGAGGCGGAGAATTTCGATCAGCTTCGCGACAGGAAGCCGGTCGAG
CCGCTCCGCTTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References