Detailed information of TA system TA193296

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1966443..1967125
• Replicon: chromosome
• Accession: NZ_CP070144
• Organism: Klebsiella oxytoca strain FDAARGOS_1331

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: I6K61_RS09075
• Protein ID: WP_064360218.1
• Coordinates: 1966443..1966763 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: I6K61_RS09080
• Protein ID: WP_064360219.1
• Coordinates: 1966784..1967125 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K61_RS09050	1962051..1962755	+	705	WP_032726119.1	DNA/RNA non-specific endonuclease	-
I6K61_RS09055	1963044..1963367	+	324	WP_023320260.1	endoribonuclease SymE	-
I6K61_RS09060	1963514..1963948	+	435	WP_023320259.1	VOC family protein	-
I6K61_RS09065	1964114..1964857	+	744	WP_049593821.1	hypothetical protein	-
I6K61_RS09070	1965203..1966336	+	1134	WP_064360217.1	hypothetical protein	-
I6K61_RS09075	1966443..1966763	-	321	WP_064360218.1	TA system toxin CbtA family protein	Toxin
I6K61_RS09080	1966784..1967125	-	342	WP_064360219.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6K61_RS09085	1967136..1967675	-	540	WP_064360220.1	DNA repair protein RadC	-
I6K61_RS09090	1967688..1968128	-	441	WP_064360221.1	antirestriction protein	-
I6K61_RS09095	1968159..1968980	-	822	WP_064360222.1	DUF945 domain-containing protein	-
I6K61_RS09100	1969100..1969573	-	474	WP_064360223.1	hypothetical protein	-
I6K61_RS09105	1969645..1970097	-	453	WP_000734321.1	hypothetical protein	-
I6K61_RS09110	1970133..1970849	-	717	WP_012543038.1	WYL domain-containing protein	-
I6K61_RS09115	1971093..1971968	-	876	WP_064360224.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1963514..2002503	38989

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-102)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12024.91 Da
• Isoelectric Point: 7.1633

>T193296 WP_064360218.1 NZ_CP070144:c1966763-1966443 [Klebsiella oxytoca]
MKTLPAINPQAATLCLSPVAVWQKLLARLLEQHYGLTLNDTPFSDETVILEHIYAGISLADAVNFLVEKYELVRIDRRGF
NWREQSPYLQVVDILRVRQAIGSSSK

Nucleotide

• Length: 321 bp

>T193296 NZ_CP092844:1274427-1274843 [Burkholderia ambifaria]
GTGTTTTTGATTGATACGAACGTCATCAGCGAGATCAGAAAGGGCAAGCGAACCAACCGCGGCGTGCGCGCGTTCTTCAG
GCAGGCGGAGGCCGACGCGAGCCCGCTTTACCTGTCGGTCGTGACGGTGGCCGAACTGCGGCGCGGCGTCGACCTGATCC
GCCATCGCGGCGATCATCCGCAGGCGTCGGCGCTCGAGGCATGGATGGCGACGATCCTGTCCGGCTATGCGTCGAACATC
CTGCCGGTCGATATCGAGACGGCCCAGATGTGGGGCCATTTGCGCGTGCCCGATCCGGCGCACGCCCTCGATCACCTGAT
CGCGGCCACCGCGCTGATCAACGATCTGACGGTCGTTACCCGCAATGTCGACGATTTCGCTCGCACCGGCGTCCGGCTGC
TGAACCCGTTCGATTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12873.37 Da
• Isoelectric Point: 5.4865

>AT193296 WP_064360219.1 NZ_CP070144:c1967125-1966784 [Klebsiella oxytoca]
MKSTDSENDSFLQWGLNRNVTPCFGARLVQEGNRLHYLVDRASITGEFSNAESLKLDEVFPHFISQMESMLTTGELNPRH
AHCFTLYHNGFTCEADTLGSYGYVYIAIYPTQR

Nucleotide

• Length: 342 bp

>AT193296 NZ_CP092844:1274204-1274440 [Burkholderia ambifaria]
ATGGCAAATCTTCTGGTGCGTAACGTGGATGACAGTATCGTTCAGAGCCTGCGCGAGCAGGCCGCGGCCAACGGCAGGAG
CGCCGAGGCCGAACATCGGGCCATTCTGGCCGCGGCGCTCGGCCGGCCGAAGCGACGGACATTCGCGCACGTGCTGATGA
GCATGCCGGACGTCGGCGAAGACGCGGATTTCCAACGTGTTCAGGATTCCGGCGAGGTCAGGCGTGTTTTTGATTGA