Detailed information of TA system TA193294

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1180457..1181076
• Replicon: chromosome
• Accession: NZ_CP070144
• Organism: Klebsiella oxytoca strain FDAARGOS_1331

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: H3N9D8
• Locus tag: I6K61_RS05540
• Protein ID: WP_004099646.1
• Coordinates: 1180858..1181076 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: I6K61_RS05535
• Protein ID: WP_004099648.1
• Coordinates: 1180457..1180831 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K61_RS05525	1175612..1176805	+	1194	WP_004099653.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
I6K61_RS05530	1176828..1179974	+	3147	WP_004099650.1	multidrug efflux RND transporter permease subunit	-
I6K61_RS05535	1180457..1180831	+	375	WP_004099648.1	Hha toxicity modulator TomB	Antitoxin
I6K61_RS05540	1180858..1181076	+	219	WP_004099646.1	hemolysin expression modulator Hha	Toxin
I6K61_RS05545	1181237..1181803	+	567	WP_004099644.1	maltose O-acetyltransferase	-
I6K61_RS05550	1181940..1182410	+	471	WP_004099643.1	YlaC family protein	-
I6K61_RS05555	1182385..1183839	-	1455	WP_017146481.1	PLP-dependent aminotransferase family protein	-
I6K61_RS05560	1183942..1184640	+	699	WP_004099639.1	GNAT family N-acetyltransferase	-
I6K61_RS05565	1184637..1184777	-	141	WP_003859006.1	type B 50S ribosomal protein L36	-
I6K61_RS05570	1184777..1185040	-	264	WP_004099638.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8640.05 Da
• Isoelectric Point: 8.9008

>T193294 WP_004099646.1 NZ_CP070144:1180858-1181076 [Klebsiella oxytoca]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Nucleotide

• Length: 219 bp

>T193294 NZ_CP092844:965128-965547 [Burkholderia ambifaria]
ATGATCCTCGTCGATACCAACGTCATTTCCGAGCCGCTGCGGCGCGAGCCGAGTGCGGCCGTGATCGAATGGCTCGATGC
GCAGAATGTCGAGACGCTGTTTCTTGCCGCGATCAGTCTCGCGGAAATGCGTTTCGGCGTGGCCGCGTTGCCGGAAGGGA
AAAGGCGGGACTGGCTGCACCAAAGCATCGAGCAGCGCGTCGTGCCGCTGTTTCGCGGCCGGATCCTGCCGTTCGACGAT
GCGGCCAGTAAAGCCTATGCGAGCCTTCGCGCGAAGGCTCGGGCCACGGGCAACGCGATGGCGTCTGCCGATGGATTTAT
CGCTGCAACGGCCGCGGCGAACGGCTTGATCGTGGCGACGCGTGACGTCGCGCCGTTCGAAGCGGCGGGTTTGCGCGTGA
TCGACCCGTGGGCACGGTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14350.09 Da
• Isoelectric Point: 4.8989

>AT193294 WP_004099648.1 NZ_CP070144:1180457-1180831 [Klebsiella oxytoca]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLVTQLDEYL
DDTFVLFSNYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT193294 NZ_CP092844:964892-965131 [Burkholderia ambifaria]
ATGCCAGTTATTACCGTTCGAAATTTGCCGGACGAGGTGCATCGCGCGCTTCGCATCCGTGCGGCGCAACATGGTCGGAG
TACCGAAGCGGAGGTGCGCGCCATCCTCGAGGAAGCCGTTCGGCCCACCGGGCGCGCGAAGCTGGGGTCGCTGCTGGCCG
AAATCGGGCGGGAGGCGGGCGGTGTCGATTTCGAGAATCCGCGTGAGAGCTCGCCAACCGCTCCGATGAGCTTCGAATGA

Structures

Toxin

• AlphaFold DB: A0A0H3H713