Detailed information of TA system TA193290

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4914192..4914976
• Replicon: chromosome
• Accession: NZ_CP070134
• Organism: Escherichia coli strain FDAARGOS_1281

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: I6K11_RS24500
• Protein ID: WP_000613626.1
• Coordinates: 4914192..4914686 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: I6K11_RS24505
• Protein ID: WP_001110447.1
• Coordinates: 4914683..4914976 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K11_RS24480 (4909385)	4909385..4910482	+	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-
I6K11_RS24485 (4910482)	4910482..4911423	+	942	WP_001522863.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
I6K11_RS24490 (4911489)	4911489..4913132	+	1644	WP_001522865.1	flagellar hook-associated protein FlgK	-
I6K11_RS24495 (4913144)	4913144..4914097	+	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
I6K11_RS24500 (4914192)	4914192..4914686	-	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
I6K11_RS24505 (4914683)	4914683..4914976	-	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
I6K11_RS24510 (4915109)	4915109..4918294	-	3186	WP_001522867.1	ribonuclease E	-
I6K11_RS24515 (4918867)	4918867..4919826	+	960	WP_000846343.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T193290 WP_000613626.1 NZ_CP070134:c4914686-4914192 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T193290 NZ_CP092842:86464-86991 [Bradyrhizobium sp. 62B]
GTGACGGTTCCGGCTTGGCATGAGGAGCCGATCGCCAAGAGCCATGATCGGGAAACCTTTGACTGTGGGGACGACGCGCT
GAACACCTTCCTGATGCGCTATGCGCGCCAGGGGCATGAGCAGAACGCGGTCAAGACCTATTGTGCGATCGCCAATGACG
CGCCGAGCCTCGTCCTCGGCTTCTACAGCGTTGCGCCGGCGTCGGTCGCTCACAAGGCCGTGGCGGCCGTCATGACCAAG
GGCCTGGCGCGCCACAAAGTGCCGGGATTCCTGCTGGCGCGGCTCGCGGTCGACAAGACGGTCGCGGGCAGGGGACTGGG
TGGCCAGCTCCTGTTGGCGGCCGCTTTGCGCTGTTTGCGGGTGACGGAAGAGATCGGCGGCGTCCTGATAGGCATTGAGG
CCAAGAACGAGCGGGCCGCGCAGTGGTATCGCGGCTTTGGCGCCGAGCCGCTGGCCTACCGGCCGCTTACGCTTGTTGCG
CCGCTTGCGACCTTCGCCGATGCGTTGCGGGCAGCCGGACGGCTCTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT193290 WP_001110447.1 NZ_CP070134:c4914976-4914683 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT193290 NZ_CP092842:86189-86467 [Bradyrhizobium sp. 62B]
ATGGCGCGCGTTGCCATCGATGACACCAACCGCATGAATCTGCGGATCAAGCCGGAGGTGAAAGCCCGGCTGATGCGCGC
GGCTGCGCTACGCCATACTGATCTGACCAGTTTTGTGACGCAGTCGGCGCTGCGCGAAGCGGACGCCGTCATTGCCGAAG
CCGATGTGATCAAGGTGCCGGAACGCGATTTTGCGCGCATCCTCGACCTCCTGGACAATCCGCCCAAGGCGAATGCCAAG
CTGCGCGCGGCCGCAGCCAGTCTGCCCAAAGATCTGTGA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6