Detailed information of TA system TA193289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 4571787..4572492
• Replicon: chromosome
• Accession: NZ_CP070134
• Organism: Escherichia coli strain FDAARGOS_1281

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: I6K11_RS22685
• Protein ID: WP_000539521.1
• Coordinates: 4572106..4572492 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: I6K11_RS22680
• Protein ID: WP_001280945.1
• Coordinates: 4571787..4572116 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K11_RS22665 (4566944)	4566944..4567855	+	912	WP_001236019.1	glutathione ABC transporter permease GsiD	-
I6K11_RS22670 (4568033)	4568033..4570381	+	2349	WP_021523051.1	EAL domain-containing protein	-
I6K11_RS22675 (4570389)	4570389..4571717	+	1329	WP_000086873.1	GGDEF domain-containing protein	-
I6K11_RS22680 (4571787)	4571787..4572116	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
I6K11_RS22685 (4572106)	4572106..4572492	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K11_RS22690 (4572718)	4572718..4574043	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
I6K11_RS22695 (4574256)	4574256..4574639	+	384	WP_000497137.1	biofilm formation regulator BssR	-
I6K11_RS22700 (4574750)	4574750..4575865	+	1116	WP_000555050.1	aldose sugar dehydrogenase YliI	-
I6K11_RS22705 (4575862)	4575862..4576488	-	627	WP_020233670.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T193289 WP_000539521.1 NZ_CP070134:c4572492-4572106 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T193289 NZ_CP092841:c4002379-4001942 [Bradyrhizobium sp. 62B]
GTGTACCTGGCCGATACCAACGTTATATCTGCGGTGGCGCCGAACCGGCCGGTGCCGACGGCGCTCGTCGAATGGATGGA
TGCACAATCCGGTTCCCTGTATTTGTCGGCCGTGACGATTGCAGAGATCGAAGACGGAATAGCAAAGCTTCGGCGCGAGA
AGGCTACGCGCAGAAGCAAAGACTTGGCCCTATGGCTGGATGCCGTCGTGCATCTCTATGGCGACCGAATCTTGGCGTTC
GACACAGCGGTCGCGCGGCTCGCCGGGCAGCTATCGGATCGGGCTCGTGGGCAGGGACATACGCCGGGGTTTGCCGATAT
CATCATCGCTGCGACGGCATGGCGGCATTCCCTGACGATCCTCTCTCGCAATATGAGGCATTTCGAACCACTAGGGGTGC
CGGCGCTCGATCCCTTCAGTAAGTTACCGCCCTCCTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT193289 WP_001280945.1 NZ_CP070134:c4572116-4571787 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT193289 NZ_CP092841:c4002625-4002383 [Bradyrhizobium sp. 62B]
ATGCGCGAAATTCAGCTTCGTGATGCAAAGGCCAATCTCTCCGCGGTTGTTGATGAAGCCCTCGATGGCAAACCAGCGGT
GATCACACGCCACGGCAAGAAGCAAGCCGTCGTCCTGAGCTACGAGGAATGGCGGCGGTTGTCGCAGGTGCCGAGCTTCG
GTCGTCTGTTGATGGCCGCGCCCCTGAGAGCGGGCGATTTACCGGCGCGCAATCGGTCCAAGCTACGCAAGACCGATTTC
TGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6