Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4149509..4150127 Replicon chromosome
Accession NZ_CP070134
Organism Escherichia coli strain FDAARGOS_1281

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6K11_RS20830 Protein ID WP_001291435.1
Coordinates 4149509..4149727 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6K11_RS20835 Protein ID WP_000344800.1
Coordinates 4149753..4150127 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K11_RS20795 (4144798) 4144798..4145370 + 573 WP_000779831.1 YbaY family lipoprotein -
I6K11_RS20800 (4145401) 4145401..4145712 - 312 WP_000409911.1 MGMT family protein -
I6K11_RS20810 (4146091) 4146091..4146444 + 354 WP_000878140.1 DUF1428 domain-containing protein -
I6K11_RS20815 (4146486) 4146486..4148036 - 1551 WP_001577872.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6K11_RS20820 (4148200) 4148200..4148670 - 471 WP_001490888.1 YlaC family protein -
I6K11_RS20825 (4148786) 4148786..4149337 - 552 WP_001522034.1 maltose O-acetyltransferase -
I6K11_RS20830 (4149509) 4149509..4149727 - 219 WP_001291435.1 HHA domain-containing protein Toxin
I6K11_RS20835 (4149753) 4149753..4150127 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6K11_RS20840 (4150673) 4150673..4153822 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
I6K11_RS20845 (4153845) 4153845..4155038 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T193288 WP_001291435.1 NZ_CP070134:c4149727-4149509 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T193288 NZ_CP092833:2423982-2424722 [Bacillus mycoides]
TTGACGATCTCTAACATTCGAATTGGTTTATTTATTCTAGTAATCGCCTTTGTAGTTCTTGTTTTCTTTTATTGGAGAAA
TGAAGAGTTGTACGAGGAGAAGAAACAACGAATTCGAAAAACTTGGTATGGTTTGTTTATAATATCAGTCACAGTTTATT
TCATGATAAAAGGAATAGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCGATGGTAATTTTTGTTGATATTGCA
TTTATTTTGACACCAAATATTTCAGAAATATGGGGCGCAAAATTCAGCGATATTGGAAAGACGGTCCAATCAATTAAAAG
GTCATTAATTGCTTCAAAAGCAAGAGGAGAAATATATACGACAATTATTCAAAATGTGAATCCAACAGCCTTTGGGACAA
TGGAATGGCAAACGGAAGAGGAATATACAAAAAGCTTAAACGCATTTTTAGATTCATATGGGGAAAAGATTGGTGCGAAA
ATTGTTGTATTTGAAGCGGTGAAGGAATTAAATACAAACTTTCGTGGTATCCGCTCTCAATTTAGTATTATTGTTCCATT
AGAACACATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGAATTATACCAGCGAAAATAGTTAGTG
ATGTTTTCATTGTTATTGATGGGAAGAAAAATAACCTTCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATTCAT
CATAGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT193288 WP_000344800.1 NZ_CP070134:c4150127-4149753 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT193288 NZ_CP092833:2424835-2424960 [Bacillus mycoides]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCTTTAATAAAAGGAGATAC
TTTGCAGCAAGTAGAAGAGTTAGAAGAAGAATTAAAAAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References