Detailed information of TA system TA193277

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1940359..1941194
• Replicon: chromosome
• Accession: NZ_CP070134
• Organism: Escherichia coli strain FDAARGOS_1281

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1C0Y405
• Locus tag: I6K11_RS10395
• Protein ID: WP_001774607.1
• Coordinates: 1940817..1941194 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: I6K11_RS10390
• Protein ID: WP_204682353.1
• Coordinates: 1940359..1940727 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K11_RS10355 (1936015)	1936015..1936695	+	681	WP_032082725.1	WYL domain-containing protein	-
I6K11_RS10360 (1936843)	1936843..1937520	+	678	WP_001097305.1	hypothetical protein	-
I6K11_RS10365 (1937526)	1937526..1937759	+	234	WP_001278287.1	DUF905 family protein	-
I6K11_RS10370 (1937858)	1937858..1938676	+	819	WP_016238867.1	DUF932 domain-containing protein	-
I6K11_RS10375 (1938939)	1938939..1939418	+	480	WP_032152717.1	antirestriction protein	-
I6K11_RS10380 (1939434)	1939434..1939910	+	477	WP_001186774.1	RadC family protein	-
I6K11_RS10385 (1939975)	1939975..1940196	+	222	WP_000692345.1	DUF987 domain-containing protein	-
I6K11_RS10390 (1940359)	1940359..1940727	+	369	WP_204682353.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6K11_RS10395 (1940817)	1940817..1941194	+	378	WP_001774607.1	TA system toxin CbtA family protein	Toxin
I6K11_RS10400 (1941191)	1941191..1941679	+	489	WP_001774608.1	DUF5983 family protein	-
I6K11_RS10405 (1941691)	1941691..1941888	+	198	WP_032152719.1	DUF957 domain-containing protein	-
I6K11_RS10410 (1941973)	1941973..1942815	+	843	WP_032152755.1	DUF4942 domain-containing protein	-
I6K11_RS10415 (1943157)	1943157..1943327	+	171	Protein_1860	IS110 family transposase	-
I6K11_RS10420 (1944138)	1944138..1945121	+	984	WP_032152721.1	KpsF/GutQ family sugar-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1900121..1942942	42821

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14218.20 Da
• Isoelectric Point: 7.8045

>T193277 WP_001774607.1 NZ_CP070134:1940817-1941194 [Escherichia coli]
MKTLPDTHVREASRCPPPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDTVNFLVEKYALIRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T193277 NZ_CP092828:2943028-2943336 [Klebsiella michiganensis]
ATGCAGTATTACGTTTATAAGAATACAGGTCGAATTGCTGCGTATCCTTACCTCCTTGATGTACAGAGTGACATTATCGG
CAAGCGCAATACTCGGGTCGTGATTCCACTGTTTCCACTGAAAAACTATAAAGGTCCACGCGCCGATCGTTTAACACCGC
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGTATCCCACATCGTGTTCTGGGTGAAGAA
GTTTGTAATCTGAATCACCAGCGCGAAGTCGTTAAAGCTTCAATGGATTTTCTTTTCGACGGGATCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13631.41 Da
• Isoelectric Point: 6.7386

>AT193277 WP_204682353.1 NZ_CP070134:1940359-1940727 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGNRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT193277 NZ_CP092828:2942789-2943025 [Klebsiella michiganensis]
ATGCCAGCCGCAAAACGCAATAAGCAGAACGTCAGCGTGACACTGGACCCAGGCCTGGTCAAACAGGCTCGTGAGGTCGG
CATCAATCTGTCTCTAACGTTGACTAACGCTCTAAAAAGCGAAATCCACAGCCTCGAAATAGCCCGCTGGCAAAAAGAAA
ATGCCCAGGCAATCGACTACCTCAATAATTTAACTGAGGAAAATGGACTTCTCTCTGATGAATACAGGACGTTCTAA

Structures

Toxin

• AlphaFold DB: A0A1C0Y405