Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 27300..27825 | Replicon | plasmid unnamed2 |
| Accession | NZ_CP070133 | ||
| Organism | Escherichia coli strain FDAARGOS_1281 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | S1PFV8 |
| Locus tag | I6K11_RS00255 | Protein ID | WP_001159871.1 |
| Coordinates | 27520..27825 (+) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | S1PPD8 |
| Locus tag | I6K11_RS00250 | Protein ID | WP_000813634.1 |
| Coordinates | 27300..27518 (+) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| I6K11_RS00245 (24337) | 24337..24978 | - | 642 | WP_000990667.1 | hypothetical protein | - |
| I6K11_RS00250 (27300) | 27300..27518 | + | 219 | WP_000813634.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| I6K11_RS00255 (27520) | 27520..27825 | + | 306 | WP_001159871.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| I6K11_RS00260 (27826) | 27826..28635 | + | 810 | WP_000016979.1 | site-specific integrase | - |
| I6K11_RS00265 (28773) | 28773..29048 | - | 276 | WP_000239529.1 | hypothetical protein | - |
| I6K11_RS00270 (29042) | 29042..29686 | - | 645 | WP_000633911.1 | AAA family ATPase | - |
| I6K11_RS00275 (29915) | 29915..30886 | + | 972 | WP_001103694.1 | plasmid segregation protein ParM | - |
| I6K11_RS00280 (30891) | 30891..31283 | + | 393 | WP_000340835.1 | plasmid partitioning/stability family protein | - |
| I6K11_RS00285 (31288) | 31288..31539 | - | 252 | Protein_36 | DUF4113 domain-containing protein | - |
| I6K11_RS00290 (31601) | 31601..32398 | - | 798 | WP_000544830.1 | IS21-like element IS21 family helper ATPase IstB | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | sul1 / qacE / aadA5 / dfrA17 / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / mph(A) / tet(B) | - | 1..151845 | 151845 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11692.49 Da Isoelectric Point: 6.4674
>T193251 WP_001159871.1 NZ_CP070133:27520-27825 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T193251 NZ_CP092795:132783-132932 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8372.34 Da Isoelectric Point: 4.7405
>AT193251 WP_000813634.1 NZ_CP070133:27300-27518 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW
Download Length: 219 bp
>AT193251 NZ_CP092795:c132738-132679 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CCE8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CQY2 |