Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 90745..91270 Replicon plasmid unnamed1
Accession NZ_CP070125
Organism Escherichia coli strain FDAARGOS_1292

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag I6K22_RS24480 Protein ID WP_001159871.1
Coordinates 90745..91050 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag I6K22_RS24485 Protein ID WP_000813634.1
Coordinates 91052..91270 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K22_RS24450 (86012) 86012..87282 + 1271 Protein_103 translesion error-prone DNA polymerase V subunit UmuC -
I6K22_RS24455 (87287) 87287..87679 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
I6K22_RS24460 (87684) 87684..88655 - 972 WP_001103694.1 plasmid segregation protein ParM -
I6K22_RS24465 (88884) 88884..89528 + 645 WP_000633911.1 AAA family ATPase -
I6K22_RS24470 (89522) 89522..89797 + 276 WP_000239529.1 hypothetical protein -
I6K22_RS24475 (89935) 89935..90744 - 810 WP_000016979.1 site-specific integrase -
I6K22_RS24480 (90745) 90745..91050 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
I6K22_RS24485 (91052) 91052..91270 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
I6K22_RS24490 (93592) 93592..94233 + 642 WP_000990667.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..114213 114213


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T193250 WP_001159871.1 NZ_CP070125:c91050-90745 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T193250 NZ_CP092794:c124607-124164 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT193250 WP_000813634.1 NZ_CP070125:c91270-91052 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT193250 NZ_CP092794:c124834-124604 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References