Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2823747..2824578 Replicon chromosome
Accession NZ_CP070124
Organism Escherichia coli strain FDAARGOS_1292

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag I6K22_RS13475 Protein ID WP_000854814.1
Coordinates 2823747..2824121 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag I6K22_RS13480 Protein ID WP_001285586.1
Coordinates 2824210..2824578 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K22_RS13435 (2819143) 2819143..2820309 + 1167 WP_001297905.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
I6K22_RS13440 (2820428) 2820428..2820901 + 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
I6K22_RS13445 (2821099) 2821099..2822157 + 1059 WP_001200890.1 FUSC family protein -
I6K22_RS13450 (2822329) 2822329..2822658 + 330 WP_000450409.1 DUF496 family protein -
I6K22_RS13455 (2822759) 2822759..2822893 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
I6K22_RS13460 (2822995) 2822995..2823141 + 147 Protein_2653 transposase domain-containing protein -
I6K22_RS13465 (2823430) 2823430..2823510 - 81 Protein_2654 hypothetical protein -
I6K22_RS13470 (2823556) 2823556..2823750 - 195 WP_000988601.1 DUF5983 family protein -
I6K22_RS13475 (2823747) 2823747..2824121 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
I6K22_RS13480 (2824210) 2824210..2824578 - 369 WP_001285586.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
I6K22_RS13485 (2824652) 2824652..2824873 - 222 WP_000692323.1 DUF987 domain-containing protein -
I6K22_RS13490 (2824942) 2824942..2825418 - 477 WP_001351157.1 RadC family protein -
I6K22_RS13495 (2825434) 2825434..2825919 - 486 WP_000213703.1 antirestriction protein -
I6K22_RS13500 (2826010) 2826010..2826831 - 822 WP_001234569.1 DUF932 domain-containing protein -
I6K22_RS13505 (2827052) 2827052..2827462 - 411 WP_000846704.1 hypothetical protein -
I6K22_RS13510 (2827478) 2827478..2828155 - 678 WP_001362823.1 hypothetical protein -
I6K22_RS13515 (2828291) 2828291..2829361 - 1071 WP_000102631.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T193234 WP_000854814.1 NZ_CP070124:c2824121-2823747 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T193234 NZ_CP092790:27675-27959 [Klebsiella pneumoniae]
ATGAAAACAAAACCAGAATTATTTGATGTAGTCGATTACCTAGAGGATGAAGAAGATATTCAGGCATATCTCAATGCCGC
GCTGGATGAAGATGATGAAAGCTACTTTTTAGCAGCACTGGGAAATATTGCCAGAGCAAGAAATATCAGCCAGCTTTCAA
AAGAAACAGGAATGAGCCGTGAGGGAATTTATAAAGCTCTTTCCGGAACAGGAAACCCATCTTTTAAAACGATCTCAAAA
ATATCTAAAGCATTAGGTTTGAAAATACACTTCGCAGGGGCTTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT193234 WP_001285586.1 NZ_CP070124:c2824578-2824210 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT193234 NZ_CP092790:27377-27673 [Klebsiella pneumoniae]
ATGATTGAAATCAGGCAAACAGAAGAAGTTAAAAAATGGCTTAAAGGATTAAAAGATAAAATAGCCAAAGCAAAAATTTT
AACACGGATTGAGCGGATGAAAGAGGGGAATTATGGAGATGTTGAGCCGATAGGTGATGGATATTCAGAGTTAAGAATCC
ATCAGGGTAAGGGTTACAGAGTTTATTTTGCCAACCGAAACAATGAAATTATTTTATTGCTTTGTGGCGGTGATAAAACA
ACACAACAAGCAGACATAAAAAAAGCAAAACAGATAGCGAAAAAATGGGGGTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References