Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2017759..2018342 Replicon chromosome
Accession NZ_CP070124
Organism Escherichia coli strain FDAARGOS_1292

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag I6K22_RS09660 Protein ID WP_000254750.1
Coordinates 2018007..2018342 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag I6K22_RS09655 Protein ID WP_000581937.1
Coordinates 2017759..2018007 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K22_RS09645 (2014098) 2014098..2015399 + 1302 WP_000046816.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
I6K22_RS09650 (2015447) 2015447..2017681 + 2235 WP_000226815.1 GTP pyrophosphokinase -
I6K22_RS09655 (2017759) 2017759..2018007 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
I6K22_RS09660 (2018007) 2018007..2018342 + 336 WP_000254750.1 endoribonuclease MazF Toxin
I6K22_RS09665 (2018414) 2018414..2019205 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
I6K22_RS09670 (2019433) 2019433..2021070 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
I6K22_RS09675 (2021158) 2021158..2022456 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T193232 WP_000254750.1 NZ_CP070124:2018007-2018342 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T193232 NZ_CP092790:25786-26052 [Klebsiella pneumoniae]
ATGAAAACCATAAGAAAAAACAAAGGCGATGTGACTTATTATTTAAGCAGAGATAATAACGATAGTTATCGTCTTATTAA
AAAAATTAAGGCGAGAGCGACTCATTTAGTTAAAGATGGACATAAAACAACCAAAATAACATTAAGTGATTTGTTATTAA
CGCATGACCAACTTTATAACTTAGATTATTCATTAAACGGGCTGAGGGCAGATGATAAAATCACAATTGAATTACTTATA
GGGGAGTTTTTTAAAAATGGAAAATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT193232 WP_000581937.1 NZ_CP070124:2017759-2018007 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT193232 NZ_CP092790:26042-26272 [Klebsiella pneumoniae]
ATGGAAAATAATGCAAAAAATATAAAAGCGTTGAGATTAAAAACAGGCTTAACACAAAAGGAAATGGCAGAAAGGTACGG
GGTAGGGATCAGAACTTGGCAAAAAAAAGAGGAAGACGGGACGCAAAGCAGTCAGAAGTTATCAAATTTTGATTTTGAAT
ACTTGTTGTTATTGGCTGGTGAGCATCCTGATTTTGTGCTGGAAAAAAGAAATAAGGAAGTTGCAAGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References